Sequences from confirmation amplicon


Confirmation sequences for line 112E05 and hit At4g26640 are shown.
Line ID 112E05
Locus At4g26640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13437073
Primers used for PCR IS04 - 8474, show primer details
   
Sequence Name 25-K015963-022-112-E05C-8409
GenBank Accession LN497219 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K015963-022-112-E05C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAAGCCATTTATCATTGGTAATTA
CTCTTTCTTTTTCTTTCATGGAGCATATCTAATTACGGTGAAGAAGAAATAGTTTGTAGT
AATATTGCTGTTTCTTGTTTAAATAAAAGCATTAACATCTTGTTGTGTATTTTTACATAA
CATTTTTGGAACTCAATACAGGTGACACAAACAAATATATATGGTGGAGATAGTTTGAAT
CAAGATCAGCTCTCTGTGAAGAAATAGGAATTATATAGAACAAGGCCTCGTCCCATTCCT
AACCTAAGAAGATCCCGTACATAATGTTGCTTACTTTTTGTTTTACGGACCCGATTGTAC
TCTCCCCATGTTGGGCGGATATGGGTAAGATGAATTGTTCAAGGACGAGATGTCACCATT
TTGAGTCTCGTTCTTTGTTTCTCTCTGGCCGTACTGATTCATACCGCTATTTAAGCTAGC
ATAGTAGGATGACATGGGACTAGCATGAACAAACCTGAAATTGACTCCATTTGGGTGAGT
TTGGTTGACAAGTTGTTGATTCTGGTGCTGATGTTCGTTGGAAGCGTGGTTAGGTCCATC
AGATGAGATTCCAACACCAAGATTGAGGCTGATGGTGTCTGTTTCATCTGGTCTGAACCG
AGGCTGGATTTCGTGATTGCTGCTAGACTTT
   
Sequence Name 26-K015963-022-112-E05C-IS04
GenBank Accession LN497691 [GenBank]
Sequencing primer gene specific primer, IS04: 5'-GATGTTCCCACTTCAAAGTCTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K015963-022-112-E05C-is04
ACAATCACGAAATCCAGCCTCGGTTCAGACCAGATGAAACAGACACCATCAGCCTCAATC
TTGGTGTTGGAATCTCATCTGATGGACCTAACCACGCTTCCAACGAACATCAGCACCAGA
ATCAACAACTTGTCAACCAAACTCACCCAAATGGAGTCAATTTCAGGTTTGTTCATGCTA
GTCCCATGTCATCCTACTATGCTAGCTTAAATAGCGGTATGAATCAGTACGGCCAGAGAG
AAACAAAGAACGAGACTCAAAATGGTGACATCTCGTCCTTGAACAATTCATCTTACCCAT
ATCCGCCCAACATGGGGAGAGTACAATCGGGTCCGTAAAACAAAAAGTAAGCAACATTAT
GTACGGGATCTTCTTAGGTTAGGAATGGGACGAGGCCTTGTTCTATATAATTCCTATTTC
TTCACAGAGAGCTGATCTTGATTCAAACTATCTCCACCATATATATTTGTTTGTGTCACC
TGTATTGAGTTCCAAAAATGTTATGTAAAAATACACAACAAGATGTTAATGCTTTTATTT
AAACAAGAAACAGCAATATTACTACAAACTATTTCTTCTTCACCGTAATTAGATATGCTC
CATGAAAGAAAAAGAAAGAGTAATTACCAATGATAAATGGCTTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT

Last Updated on 06.12.2018 16:11