Sequences from confirmation amplicon


Confirmation sequences for line 114A12 and hit At4g32980 are shown.
Line ID 114A12
Locus At4g32980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15916018
Primers used for PCR MR50 - 3144, show primer details
   
Sequence Name 50-K016292-022-114-A12C-3144
GenBank Accession LN507012 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K016292-022-114-A12C-3144
GGATGAGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTT
CATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAAC
TATCAGTGTTTGAAGAAGATCCAAGAGATGGGTTTTCTTTGCTTCTAATGCTCTCCTTTG
AAATGTAGATCCGAAACCCGCCTCCGAGTTATTAGAATCACCATCAAGAAACTCAGTTAT
ATTCTCAAAACGTGAAGTAAAGGCTGAACTAGCAGCTCCTGACTCGGTTCCTCGAGATGA
ATAATCGAGCGAGTATGCGGCGAAATGAGATAGTATTTCTTGAACAGAGTGAAGGTATTT
TGAGCCAA
   
Sequence Name 51-K016292-022-114-A12C-MR50
GenBank Accession LN507338 [GenBank]
Sequencing primer gene specific primer, MR50: 5'-TTTGGCTCAAAATACCTTCACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K016292-022-114-A12C-mr50
GAAATACTATCTCATTTCGCCGCATACTCGCTCGATTATTCATCTCGAGGAACCGAGTCA
GGAGCTGCTAGTTCAGCCTTTACTTCACGTTTTGAGAATATAACTGAGTTTCTTGATGGT
GATTCTAATAACTCGGAGGCGGGTTTCGGATCTACATTTCAAAGGAGAGCATTAGAAGCA
AAGAAAACCCATCTCTTGGATCTTCTTCAAACACTGATAGTTTAAACCGAAGGCGGGAAA
CGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGA
GGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATC
ACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCT
CGTGGGTGGGGGT
Primers used for PCR FI66 - 3144, show primer details
   
Sequence Name 56-K035345-022-114-A12C-FI66
GenBank Accession LN509026 [GenBank]
Sequencing primer gene specific primer, FI66: 5'-ACTTCTTGTCAAGCCACTTCGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K035345-022-114-A12C-FI66
CTCGATCTTTCGGGTACTGCATTAATAAAACATAACTTTGCTTATAAAATCGAGTTAACA
ATGTCATCATCATAAAGTAGATAGTTAAGAAGAGTAAGGATTGTTACGGGTGAAGGAAGT
TTTGGAACATCCAATTCCGTAGAACCGAAACAGATTTCTCAGGCAAACCTCGTTGAGGTC
TCCAAATCTGATGGTTCTTTCGTTTCAGCTGCTGAAGAAGGCAATGCTGGTGGAACATAG
AGGTTTCTTGAGTCTTGTCTTTGCCTCTCTCCAATACAGATCCCATAGAGATTATCTTCT
TGCAGATTCTCTCTCTCAGGTTCTTGTATAAAAAGGAAACGGTTTGGAGGGCAAACCGGG
TGTGTAACTGTGGATCTAACTCGGTTGCAGCATGGAACGCTGATATAACCGTATGAATCT
CATCTACGCAATGACTATATCGATCATCCACCTGAAGAGAAATATCAACTCATAAAACTT
CATCATATATATCAAACCATTTTTTTAACTTATGCAAAAAAAGTGAATGAATATATTCAA
ACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCT
CTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTG
TGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGT
TGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG
   
Sequence Name 08-K035345-022-114-A12C-3144
GenBank Accession LN489642 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K035345-022-114-A12C-3144
TATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGG
TACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAATATA
TTCATTCACTTTTTTTGCATAAGTTAAAAAAATGGTTTGATATATATGATGAAGTTTTAT
GAGTTGATATTTCTCTTCAGGTGGATGATCGATATAGTCATTGCGTAGATGAGATTCATA
CGGTTATATCAGCGTTCCATGCTGCAACCGAGTTAGATCCACAGTTACACACCCGGTTTG
CCCTCCAAACCGTTTCCTTCTTATACAAGAACCTGAGAGAGAGAATCTGCAAGAAGATAA
TCTCTATGGGATCTGTATTGGAGAGAGGCAAAGACAAGACTCAAGAAACCTCTATGTTCC
ACCAGCATTGCCTTCTTCAGCAGCTGAAACGAAAGAACCATCAGATTTGGAGACCTCAAC
GAGGTTTGCCTGAGAAATCTGTTTCGGTTCTACGGAATTGGATGTTCCAAAACTTCCTTC
ACCCGTAACAATCCTTACTCTTCTTAACTATCTACTTTATGATGATGACATTGTTAACTC
GATTTTATAAGCAAAGTTATGTTTTATTTAATGCAGTTACCCGAAAGATTCGGAGAAACA
TCTTCTAGCTATACGAAGTGGCTTGACAAGAAG

Last Updated on 06.12.2018 16:11