Sequences from confirmation amplicon


Confirmation sequences for line 114A12 and hit T22O13 are shown.
Line ID 114A12
Locus T22O13 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11640632
Primers used for PCR PH81 - 8474, show primer details
   
Sequence Name 34-K017627-022-114-A12C-PH81
GenBank Accession LN501221 [GenBank]
Sequencing primer gene specific primer, PH81: 5'-ACGTCTAGCTTTATTTCGGTGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K017627-022-114-A12C-PH81
GGGAACTTTCCGAGGAATCAGGCAGATAATGGCATCACCAAAAAATAATGTCTAAATGTA
ATCTATTAATAAAATCCAAATTTTATATTAGTATTCGAAATTATTTTCTAGATACATTAC
TATGTCAAAATGTGTTTTAACTATGGATGGATGCTTAATTCGAAGAGTAATGAATTTCCG
GGAATTGTGGACCATGTGGTCTATTTTCCAATGTACCATTACTATCTTCAAACACAAGTA
CTGACAACACAAAGAAAACATAAGAGAAAATATGATTTAGAAAACTATATGAAAGGAATA
GGGAAATTTTGAAGATAAAGGCGGAACCAGGCAAATAAACTTTATTTATTTTGGCTGATA
AATTTTTAAACATAGATTTGCATTGCCATGTACATTTTTATGATTTTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 38-K017627-022-114-A12C-8409
GenBank Accession LN502816 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K017627-022-114-A12C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAAAATCATAAAAATGTACATGGCAATG
CAAATCTATGTTTAAAAATTTATCAGCCAAAATAAATAAAGTTTATTTGCCTGGTTCCGC
CTTTATCTTCAAAATTTCCCTATTCCTTTCATATAGTTTTCTAAATCATATTTTCTCTTA
TGTTTTCTTTGTGTTGTCAGTACTTGTGTTTGAAGATAGTAATGGTACATTGGAAAATAG
ACCACATGGTCCACAATTCCCGGAAATTCATTACTCTTCGAATTAAGCATCCATCCATAG
TTAAAACACATTTTGACATAGTAATGTATCTAGAAAATAATTTCGAATACTAATATAAAA
TTTGGATTTTATTAATAGATTACATTTAGACATTATTTTTTGGTGATGCCATTATCTGCC
TGTATTCCTCGGAAAGCTTCCCATCACACACCGAAATAAAGTTAGACG

Last Updated on 06.12.2018 16:11