Sequences from confirmation amplicon


Confirmation sequences for line 116C06 and hit At2g26510 are shown.
Line ID 116C06
Locus At2g26510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11275236
Primers used for PCR RK06 - 8474, show primer details
   
Sequence Name 22-K014583-022-116-C06C-8409
GenBank Accession LN495885 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K014583-022-116-C06C-8409
CTGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAATATGGACATATTGCTGA
TATAATATTTCTACTATATAGTGTTTCTTTTTCTTAAATGTGATGTCGCGGTGAATTGTG
GTGCAGTTCAACGATATACTTAATACGATATTTGCTTCGGCTCCGTTGGTGGCGACCATT
CTTGCGACCATACTAGATAACACGTTGGAAGCAAGACATGCAAGTGACGACGCAAGAGGA
ATCCCGTGGTGGAAGCCCTTCCAGCACAGGAACGGAGACGGCAGGAACGATGAGTTCTAT
AGTATGCCCCTTAGAATCAACGAGTTAATGCCGACACGGTTCCTTTGAAGACTGCCCCTG
AACGTTTCTTCTGTATTTGGAAATGTAAGATATGATTATGTGCATACCTTGTAGCTTCAT
TGGGGAAAAATTGAGTCCAGTGGATACAAATGAACATAGGCCTTTGATGGAAAAAGCTAT
TTTTTTGCAAACTATATAACTTGTGTTACTAGATTACTTTGCCTTTCATATCATTAGCAT
ACCTTTTGAACAAGTGCTTTATACCACATATACAAACAATTTTTGTTCTTTCATTGTTTC
TTAGAGCAGCTCCAATGCAG
   
Sequence Name 23-K014583-022-116-C06C-RK06
GenBank Accession LN496341 [GenBank]
Sequencing primer gene specific primer, RK06: 5'-AGAACCTTGCATTGGAGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K014583-022-116-C06C-RK06
TGAAAGAACAATAAATTGTTTGTATATGTGGTATAAAGCACTTGTTCAAAAGGTATGCTA
ATGATATGAAAGGCAAAGTAATCTAGTAACACAAGTTATATAGTTTGCAAAAAAATAGCT
TTTTCCATCAAAGGCCTATGTTCATTTGTATCCACTGGACTCAATTTTTCCCCAATGAAG
CTACAAGGTATGCACATAATCATATCTTACATTTCCAAATACAGAAGAAACGTTCAGGGG
CAGTCTTCAAAGGAACCGTGTCGGCATTAACTCGTTGATTCTAAGGGGCATACTATAGAA
CTCATCGTTCCTGCCGTCTCCGTTCCTGTGCTGGAAGGGCTTCCACCACGGGATTCCTCT
TGCGTCGTCACTTGCATGTCTTGCTTCCAACGTGTTATCTAGTATGGTCGCAAGAATGGT
CGCCACCAACGGAGCCGAAGCAAATATCGTATTAAGTATATCGTTGAACTGCACCACAAT
TCACCGCGACATCACATTTAAGAAAAAGAAACACTATATAGTAGAAATATTATATCAGCA
ATATGTCCATATTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
Primers used for PCR RK88 - 3144, show primer details
   
Sequence Name 20-K116785-0022-116-C06C-RK88
GenBank Accession LN495378 [GenBank]
Sequencing primer gene specific primer, RK88: 5'-TAACACTGGTCATGGAAAATGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116785-0022-116-C06C-RK88
TTTTAAGGGATTTTGTAACAAAAGGATTGTATTATTGTTTCCTTTTAAGAATTGATTTGT
CTCATTTCCATTAACAATGAATCATTTTACAGATGAGACGGTTGTACTGGCTTTCCAGCA
TTACATCGTTATGCTCGGGACTACTGTTTTGATTGCCAACACACTAGTGTCACCAATGGG
TGGAGATCCTGTATGTGATGTTTTTGGTTTTTGGATCCGGTTGTCTAATATTTATGTTAT
TATATGATTCTGAAATCGACCGTTTGGTATTTTGAACGATAATAGGGTGATAAAGCGCGG
GTTATCCAGACTATATTGTTTATGTCGGGCATAAACACGTTGCTACAAACGCTTATCGGG
ACAAGGCTTCCCACGGTTATGGGAGTATCGTTCGCGTATGTTCTTCCCGTATTGTCTATA
ATCAGAGATTACAACAATGGTCAATTCGATTCCGAGAAACAGGTTAAAGCTCGTTTTATA
CTTGCGCGTTTATCATGTCTTTGTAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCC
CCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCG
AAGGATAGTGGGATTGTGCGTCATCCCTTACGTCA
   
Sequence Name 01-K116784-0022-116-C06C-3144
GenBank Accession LN486742 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116784-0022-116-C06C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTACAAAGACATGATA
AACGCGCAAGTATAAAACGAGCTTTAACCTGTTTCTCGGAATCGAATTGACCATTGTTGT
AATCTCTGATTATAGACAATACGGGAAGAACATACGCGAACGATACTCCCATAACCGTGG
GAAGCCTTGTCCCGATAAGCGTTTGTAGCAACGTGTTTATGCCCGACATAAACAATATAG
TCTGGATAACCCGCGCTTTATCACCCTATTATCGTTCAAAATACCAAACGGTCGATTTCA
GAATCATATAATAACATAAATATTAGACAACCGGATCCAAAAACCAAAAACATCACATAC
AGGATCTCCACCCATTGGTGACACTAGTGTGTTGGCAATCAAAACAGTAGTCCCGAGCAT
AACGATGTAATGCTGGAAAGCCAGTACAACCGTCTCATCTGTAAAATGATTCATTGTTAA
TGGAAATGAGACAAATCAATTCTTAAAAGGAAACAATAATACAATCCTTTTGTTACAAAA
TCCCTTAAAAATGGCATTTCTTGTAATGGTAAAACACTAATTGCATTTCATTTTCC

Last Updated on 06.12.2018 16:11