Sequences from confirmation amplicon


Confirmation sequences for line 116E01 and hit At3g23210 are shown.
Line ID 116E01
Locus At3g23210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8284233
Primers used for PCR H34a - 8474, show primer details
   
Sequence Name 09-K014708-022-116-E01C-8409
GenBank Accession LN489956 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K014708-022-116-E01C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAACAAAGAAAAGTAAAGAG
CAAGGTATTCAAATTCTTAAAAGACTACCTCTTCCCCGAACATTCTTCCTCAAACTCTTC
CTTAACAGCTCCAACAGATGACGAAGAATTGATTTCCACTACACCACTAAACACCTGTCC
TTGTTGCTGCTGCAAATGAGGCACTGCAGAATCGTCGTTTTGTCCTCTAAAGCCACAGAC
TTGATGTGTCTCTTGCTGCAAAACTCCACTAAACGATGCAGCCAAATCCAATTGTGCTGA
TCCAATGCTACTACTATAAACAACTCCATTGTTTCCTCCTCTAAAGCTACTACAAACTTG
CTCAAGATTTCCATTAAAAGAATCAAATTGTTCTTGTTGTGGCTGCATCAGATTCACACC
ACAAGACCCAAAATCCTGAAATATGTTATCTTCATTTGTTTGCATATATCCATAAGGATC
TTCTACTCCATTGCTACAGAATCAAGGTAACAACAATCTCCATCAACAACAATAACGACC
GCTAGAAAAACTTTTCATATTCAAAATCGAAACTTTTGCTAAAATTGGATTGAAATTTGG
AATTGTTAAGAAACCCAAATAACAAAAGGCACAATACAACACAATTCAACAATAAAAATC
AAACCTTTGATCAAAACAAAGAGCAGCGAGAAGATCATCATCGTCTTC
   
Sequence Name 10-K014708-022-116-E01C-H34a
GenBank Accession LN490421 [GenBank]
Sequencing primer gene specific primer, H34a: 5'-TCCATCAATCGAAGACGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K014708-022-116-E01C-H34a
AGATCTTCTCGCTGCTCTTTGTTTTGATCAAAGGTTTGATTTTTATTGTTGAATTGTGTT
GTATTGTGCCTTTTGTTATTTGGGTTTCTTAACAATTCCAAATTTCAATCCAATTTTAGC
AAAAGTTTCGATTTTGAATATGAAAAGTTTTTCTAGCGGTCGTTATTGTTGTTGATGGAG
ATTGTTGTTACCTTGATTCTGTAGCAATGGAGTAGAAGATCCTTATGGATATATGCAAAC
AAATGAAGATAACATATTTCAGGATTTTGGGTCTTGTGGTGTGAATCTGATGCAGCCACA
ACAAGAACAATTTGATTCTTTTAATGGAAATCTTGAGCAAGTTTGTAGTAGCTTTAGAGG
AGGAAACAATGGAGTTGTTTATAGTAGTAGCATTGGATCAGCACAATTGGATTTGGCTGC
ATCGTTTAGTGGAGTTTTGCAGCAAGAGACACATCAAGTCTGTGGCTTTAGAGGACAAAA
CGACGATTCTGCAGTGCCTCATTTGCAGCAGCAACAAGGACAGGTGTTTAGTGGTGTAGT
GGAAATCAATTCTTCGTCATCTGTTGGAGCTGTTAAGGAAGAGTTTGAGGAAGAATGTTC
GGGGAAGAGGTAGTCTTTTAAGAATTTGAATACCTTGCTCTTTACTTTTCTTTGTTCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG

Last Updated on 06.12.2018 16:11