Sequences from confirmation amplicon


Confirmation sequences for line 117H08 and hit At4g23430 are shown.
Line ID 117H08
Locus At4g23430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12230686
Primers used for PCR DL12 - 3144, show primer details
   
Sequence Name 24-K017404-022-117-H08C-DL12
GenBank Accession LN496794 [GenBank]
Sequencing primer gene specific primer, DL12: 5'-ATTATGGCATGTCCTTTTATGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K017404-022-117-H08C-DL12
GACAACATCGAGCTGCAATTCGCAACCAATCATTTAGGTGAGTAGGAGAAGTAAACCAAC
ATTATGTGGCTTTGTTCTAAAGTTATATACTTAAGTTAACATCATCATTAGTTTTTAGTC
AGTGGCATGGAATTTGTGATCCACTTTATTTCAGGTCATTTTCTGTTGACTAAACTTCTG
CTAGATACAATGAAGAGCACATCACGTGAAAGCAAACGAGAAGGAAGAATTGTTAACCTA
TCATCAGAAGCTCATCGGTTCTCGTATCCAGAAGGAGTTCGTTTTGATAAGATCAATGAC
AAGTCAAGGTACTCATGTAAATAGATCAATTGTAAATGAAATGAACTTCCTTATATAGAG
GAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
CATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTC
GTGGGTGGGGG
   
Sequence Name 28-K017404-022-117-H08C-3144
GenBank Accession LN498635 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K017404-022-117-H08C-3144
GGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATT
TCATTTACAATTGATCTATTTACATGAGTACCTTGACTTGTCATTGATCTTATCAAAACG
AACTCCTTCTGGATACGAGAACCGATGAGCTTCTGATGATAGGTTAACAATTCTTCCTTC
TCGTTTGCTTTCACGTGATGTGCTCTTCATTGTATCTAGCAGAAGTTTAGTCAACAGAAA
ATGACCTGAAATAAAGTGGATCACAAATTCCATGCCACTGACTAAAAACTAATGATGATG
TTAACTTAAGTATATAACTTTAGAACAAAGCCACATAATGTTGGTTTACTTCTCCTACTC
ACCTAAATGATTGGTTGCGAATTGCAGCTCGATGTTGTCCTTAGAGAGCATAAAAGGACA
TGCCATAA
Primers used for PCR FL03 - 8474, show primer details
   
Sequence Name 40-K028350-022-117-H08C-8409
GenBank Accession LN503590 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K028350-022-117-H08C-8409
CCATTTTACAATGGTGAACCAAATAGTAATTCTTCTATCAAAATCTAACATTCTTCCTTG
GTTTGGTCTCCGTTGCAGTTACAGTAGTATGCGAGCTTATGGACAATCTAAACTCTGCAA
TGTGTTGCACGCCAACGAGCTTACAAAGCAACTGAAGGTAACTAGAAAAACAAGAGATCT
ATCAAATCAAAATCTATGTTTCCATGGTTATATTGATTCAAATCTGGTATGAATAAAATA
GGAAGATGGAGTCAATATAACAGCAAATTCGCTTCATCCAGGAGCCATTATGACTAACCT
CGGGCGCTACTTCAACCCCTATTTGGCTGGTAACTTCTTTTCTCTTATTCCACTATCATC
ACCCCATACTTCACAAGGTTACACACAAGTAAACAAACGCATACATATACTTCTTCTGAT
CCTCTCTGGCATTGGCTTGATGCAGTAGCGGTTGGTGCA
   
Sequence Name 40-K028350-022-117-H08C-FL03
GenBank Accession LN503591 [GenBank]
Sequencing primer gene specific primer, FL03: 5'-ATATATTTAGCCACTGCACCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K028350-022-117-H08C-FL03
ATGTATGCGTTTGTTTACTTGTGTGTAACCTTGTGAAGTATGGGGTGATGATAGTGGAAT
AAGAGAAAAGAAGTTACCAGCCAAATAGGGGTTGAAGTAGCGCCCGAGGTTAGTCATAAT
GGCTCCTGGATGAAGCGAATTTGCTGTTATATTGACTCCATCTTCCTATTTTATTCATAC
CAGATTTGAATCAATATAACCATGGAAACATAGATTTTGATTTGATAGATCTCTTGTTTT
TCTAGTTACCTTCAGTTGCTTTGTAAGCTCGTTGGCGTGCAACACATTGCAGAGTTTAGA
TTGTCCATAAGCTCGCATACTACTGTAACTGCAACGGAGACCAAACCAAGGAAGAATGTT
AGATTTTGATAGAAGAATTACTATTTGGTTCACCATTAGTAAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11