Sequences from confirmation amplicon


Confirmation sequences for line 118C04 and hit At1g75940 are shown.
Line ID 118C04
Locus At1g75940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28513857
Primers used for PCR VL10 - 8474, show primer details
   
Sequence Name 11-K017368-022-118-C04C-VL10
GenBank Accession LN490897 [GenBank]
Sequencing primer gene specific primer, VL10: 5'-ATATCAAGGACAAATACGGCAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K017368-022-118-C04C-vl10
CTCATGATCACCGAGAATGGTTAGAAAAACGTTTATAATCTAATTTAGTCTCAAAATCAT
ATAATCGATTTCTATATATCTTTCTTTTCTTTACTTGTATATAAGTATTTGAGTTTTGAA
TATGTTTGACGTAAGGATACGGAGAGGATCTAGGTGAGCAAGACACAAGCCTTGTAGTGG
CGCTGTCAGATCAGCACAGAACATACTATATCCAGAAACATCTCTTGAGCTTGCACGAAG
CAATTTGGTATGTTAAAAAATCATATTTTGAGTTTTAATTCGATGAAAATATGCCAATTA
TTTTCTTCAGATGGACTAATGTTTTTTTTGTTCGTGTGTTTTAATGCAGCGATGATAAAG
TAAACGTTACAGGGTATTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 15-K017368-022-118-C04C-8409
GenBank Accession LN492747 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K017368-022-118-C04C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAAATACCCTGTAACG
TTTACTTTATCATCGCTGCATTAAAACACACGAACAAAAAAAACATTAGTCCATCTGAAG
AAAATAATTGGCATATTTTCATCGAATTAAAACTCAAAATATGATTTTTTAACATACCAA
ATTGCTTCGTGCAAGCTCAAGAGATGTTTCTGGATATAGTATGTTCTGTGCTGATCTGAC
AGCGCCACTACAAGGCTTGTGTCTTGCTCACCTAGATCCTCTCCGTATCCTTACGTCAAA
CATATTCAAAACTCAAATACTTATATACAAGTAAAGAAAAGAAAGATATATAGAAATCGA
TTATATGATTTTGAGACTAAATTAGATTATAAACGTTTTTCTAACCATTCTCGGTGATCA
TGATTTCAGGATTGCCGTATTTG

Last Updated on 06.12.2018 16:11