Sequences from confirmation amplicon


Confirmation sequences for line 118G07 and hit At4g28530 are shown.
Line ID 118G07
Locus At4g28530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14094317
Primers used for PCR MZ16 - 8474, show primer details
   
Sequence Name 15-K017255-022-118-G07C-MZ16
GenBank Accession LN492746 [GenBank]
Sequencing primer gene specific primer, MZ16: 5'-CAAAGTCCCCAAAAGTCAAAATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K017255-022-118-G07C-MZ16
AGATAAGAAAAATAAAAAGATGACAACAGGATTCTCGGTCCCCTTTTAAAAAAGCTTCCT
TAACTTCTCAAGGTATATCATCAAACAAAGAACCACAAACAAATAATGAAAATCACTAGA
AACATACAAAGAACACACATATATATCTATATATATATATATCATGTTTTGATATGATGT
AAGTGCACCTATAATTATTCATTAAAATTTACCATCTGATTTACGAGTATAACGACAGCT
GTGATTTTGCATATTTTGCTGCTTTTGTACAGCCTCCAGATCTTTATTCTATAATTGCAA
ACATATTATTAAAAAGCTAGCATGTATCTCAACTAATCATGATTCATGACACTCCGGATA
ATGTTTACACTAATATTAATGCAATTATGATTTATGATCATAATTAATGAATCAGTCTAA
AATCTAGCATTTATGTGAACTCATCACCCGTTTCACAATTAAAAGATATTTGATCTTTCA
TTCATTTTCTAGAATCACGAACAAGAAGATTCGGTTTGTAAGTATAAGCATATCATAAAT
CTGTAAATATGCAAATGCGTAGATATGTATTAAGTTGACAAACATAATTAGACACATAAG
TAAACATCATCTAATTGTAATAATACTAAAAAGTTTATAGGTTAAGATTTACAATAACAA
TTAACAGACGCGGGATGATAATATCTGATTTCAAGAATTAGTTTTCCTTCTTTATGTTCA
ATTAATTTCAGGAAATATTGTT
   
Sequence Name 16-K017255-022-118-G07C-8409
GenBank Accession LN493212 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K017255-022-118-G07C-8409
GATTCTCCGACATGAAGCCATTTACAATTGAATATATTCATGGCTTCATGTAGGTTATAT
GAATTTGTTTAACTATCTTTTCACTCCTGGCTTTTCTTGGGTTTCAAATTGCTTTCGTAA
AGATTCTTGAAACTTTCCATTGGAAAACAATATTCCTGAAATTAATTGAACATAAAGAAG
AAAACTAATTCTTGAAATCAGATATTATCATCCGCGTCTGTTAATTGTTATTGTAAATCT
TAACCTATAAACTTTTTAGTATTATTACAATTAGATGATGTTTACTTATGTGTCTAATTA
TGTTTGTCAACTTAATACATATCTACGCATTTGCATATTTACAGATTTATGATATGCTTA
TACTTACAAACCGAATCTTCTTGTTCGTGATTCTAGAAAATGAATGAAAGATCAAATATC
TTTTAATTGTGAAACGGGTGATGAGTTCACATAAATGCTAGATTTTAGACTGATTCATTA
ATTATGATCATAAATCATAATTGCATTAATATTAGTGTAAACATTATCCGGAGTGTCATG
AATCATGATTAGTTGAGATACATGCTAGCTTTTTAATAATATGTTTGCAATTATAGAATA
AAGATCTGGAGGCTGTACAAAAGCAGCAAAATATGCAAAATCACAGCTGTCGTTATACTC
GTAAATCAGATGGTAAATTTTAATGAATAATTATAGGTGCACTTACATCATATCAAAACC
TGATATATATATATATAGATATATA

Last Updated on 06.12.2018 16:11