Sequences from confirmation amplicon


Confirmation sequences for line 120D06 and hit At3g21820 are shown.
Line ID 120D06
Locus At3g21820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7689423
Primers used for PCR IN41 - 3144, show primer details
   
Sequence Name 33-K033707-022-120-D06C-3144
GenBank Accession LN500898 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K033707-022-120-D06C-3144
ATCCACATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTGCTTT
GATCTCTGGCAGAGACTTGGTGGTAGCATCACCAGTAGAGGACTATTTCTTGTATATTGA
TGATCTTCCTGATGCAGAAAAGGTCTGAACAGAGTAATGCTTTCCACCTGTTTGTTCTTT
GACCAAAATCTATCGACGCTTACCAGTTTCTTATTCTCATTATGATAGGAAGAAACCGAG
GAAATCACAAGACCGTTTCTAGATGCTCTTGGTGATGAGTACTCGGACTGTTGCCAAGGT
TAGAAATGAAGGGAACATTGTAACCATAAAACAGAGGAGTTATGTAACTTGTAACCAAGA
TTTGACATTATTACATCATCTGTATTTGTGTTTAGGAACGGCCTTCTTCCCTCTGCAAAG
CTGTATGAACCATTCTTGTTGCCCTAATGCAAAAGCCTTCAAAAGAGAAGAGGTATCCTT
CTTACATCTTCGTCCTCTTTATGGCCTACAAATCTTAAGGATCGAAGATTTTTCATCAAC
ACTTTCAGACTGAATCCAAGTGTAACTCGTTTGTCTTGTGGGTTTTGCAGGACAGAGACG
GACAACAGTTGATAA
   
Sequence Name 33-K033707-022-120-D06C-IN41
GenBank Accession LN500899 [GenBank]
Sequencing primer gene specific primer, IN41: 5'-AATTATCACTGCTTGTCCGTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K033707-022-120-D06C-IN41
ACGAGTTACACTTGGATCAGTCTGAAAGTGTTGATGAAAAATCTTCGATCCTTAAGATTT
GTAGGCCATAAAGAGGACGAAGATGTAAGAAGGATACCTCTTCTCTTTTGAAGGCTTTTG
CATTAGGGCAACAAGAATGGTTCATACAGCTTTGCAGAGGGAAGAAGGCCGTTCCTAAAC
ACAAATACAGATGATGTAATAATGTCAAATCTTGGTTACAAGTTACATAACTCCTCTGTT
TTATGGTTACAATGTTCCCTTCATTTCTAACCTTGGCAACAGTCCGAGTACTCATCACCA
AGAGCATCTAGAAACGGTCTTGTGATTTCCTCGGTTTCTTCCTATCATAATGAGAATAAG
AAACTGGTAAGCGTCGATAGATTTTGGTCAAAGAACAAACAGGTGGAAAGCATTACTCTG
TTCAGACCTTTTCTGCATCAGGAAGATCATCAATATACAAGAAATAGTCCTCTACTGGTG
ATGCTACCACCAAGTCTCTGCCAGAGATCAAAGCACACTGATAGTTTAAACCGAAGGCGG
GAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATA
TAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGA
TATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGC
TCCTCGTGG

Last Updated on 06.12.2018 16:11