Sequences from confirmation amplicon


Confirmation sequences for line 121G06 and hit At1g22150 are shown.
Line ID 121G06
Locus At1g22150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7820626
Primers used for PCR JD04 - 8474, show primer details
   
Sequence Name 05-K024722-022-121-G06C-8409
GenBank Accession LN488190 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K024722-022-121-G06C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATTTACTGATTTCAAGAATCGCT
GTCCTAGGTCTCGTAACTTGCAAAAGTATCTTGGCAAAAGATATCCCCACCTAAAACATT
AACACCCAAAAACATCAGCCAAGAAAACTGCAAAACCTATCAATTCACACATATGGTACT
GTTTTCTTGGGACTTACGGCTATTAGAAGGCCAATCTCTACGGATACAAAGATGACACCA
AAAAAAGCCCCCATACAAGCAACAAAATCGAGCTTATCGATCTTGAAGATCAAAATGGTA
GCATTAACGTCAACCAAAGGAATCACAGCGTTGATGATGATCGCTGCAAGAATTGCGTTG
GGTGTGTATTTGAAAAGAGGAGTGAGGAAGAGAAGCCTTAAAA
   
Sequence Name 06-K024722-022-121-G06C-JD04
GenBank Accession LN488640 [GenBank]
Sequencing primer gene specific primer, JD04: 5'-TCTTAACGCTTCTCTTCCTCACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K024722-022-121-G06C-JD04
GCTCTTTTCAATACACACCCAACGCAATTCTTGCAGCGATCATCATCAACGCTGTGATTC
CTTTGGTTGACGTTAATGCTACCATTTTGATCTTCAAGATCGATAAGCTCGATTTTGTTG
CTTGTATGGGGGCTTTTTTTGGTGTCATCTTTGTATCCGTAGAGATTGGCCTTCTAATAG
CCGTAAGTCCCAAGAAAACAGTACCATATGTGTGAATTGATAGGTTTTGCAGTTTTCTTG
GCTGATGTTTTTGGGTGTTAATGTTTTAGGTGGGGATATCTTTTGCCAAGATACTTTTGC
AAGTTACGAGACCTAGGACAGCGATTCTTGAAATCAGTAAATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11