Sequences from confirmation amplicon


Confirmation sequences for line 122C01 and hit At2g31660 are shown.
Line ID 122C01
Locus At2g31660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13468820
Primers used for PCR CK53 - 3144, show primer details
   
Sequence Name 15-K018474-022-122-C01C-CK53
GenBank Accession LN492757 [GenBank]
Sequencing primer gene specific primer, CK53: 5'-AAACTTCTTCAAATACCTCTGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K018474-022-122-C01C-CK53
CCAAATAGGATGAGAATATAGATGCTTCAGTGTCAATTATTAAATAGAAATAGCTAGAAA
AGGAAATTAATAAACTTAAGATCGTGATGCTACAACACAACACAAGGCTGACAGAACAAA
AAAATGGATTATACAATTCTGAAAAAAAAAACCATACTAATGTAATGGACCCAAATGGCT
AGTTGGCTACCAAATCAGTCCAAATGTACATCCTCTGGACACAACTATAACTTAATTATA
AATGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATA
GAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATA
TCACATCAATCCACTTGCTTTGAAAACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTC
CTCGTGGGTGGGGGTC
   
Sequence Name 16-K018474-022-122-C01C-3144
GenBank Accession LN493226 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K018474-022-122-C01C-3144
CGTAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGT
TCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCATTTATAATTAAGTTA
TAGTTGTGTCCAGAGGATGTACATTTGGACTGATTTGGTAGCCAACTAGCCATTTGGGTC
CATTACATTAGTATGGTTTTTTTTTTCAAAATTGTATAATCCATTTTTTTGTTCTGTCAG
CCTTGTGTTGTGTTGTAGCATCACGATCTTAAGTTTATTAATTTCCTTTTCTAGCTATTT
CTATTTAATAATTGACACTGAAGCATCTATTTTCTCATCCTATTTGCTATTGGCAGAGGT
ATTTGA
Primers used for PCR FL05 - 8474, show primer details
   
Sequence Name 43-K028351-022-122-C01C-8409
GenBank Accession LN504719 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K028351-022-122-C01C-8409
ACATATCCCAAACTATATGAGTATACCTTGGCCATCAGTGGTCAACATTTTCTGCATTAT
TGGAAGTATAGTTGGTTCTATCTCAACAAACAGCTGCGGGAGACTGCTAACAGATTCAAG
GATTGTACTTATGGCACGCAAACAACCAACGGCAGCTAAAGCTCCCATGTCATCAGAGTC
GTCATTGGCTTCTGACGTGTTTAGACATCTCCAAAACGCAGCCGCCTGAACAAAAGCAAA
GGCAGAAATACATATTTATTACCAAGTTTGAGTATTCAAGAAATATCAACTCAGAGAACC
AATTTTTCTTACCAGATTTTGACATAATCCAAAAGCAAAAGGAGCCATCTCTTCGCCAAA
CTTATCTACGATGGTCTCCAAAGTAAAAACAAGGTCCTCATTCTCCTACCT
   
Sequence Name 43-K028351-022-122-C01C-FL05
GenBank Accession LN504720 [GenBank]
Sequencing primer gene specific primer, FL05: 5'-TGAGGTAGAGAATGAGGACCTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K028351-022-122-C01C-FL05
GGTAAGAAAAAATTGGTTTCTCTGAGTTGATATTTCTTGAATACTCAAACTTGGTAATAA
ATATGTATTTCTGCCTTTGCTTTTGTTCAGGCGGCTGCGTTTTGGAGATGTCTAAACACG
TCAGAAGCCAATGACGACTCTGATGACATGGGAGCTTTAGCTGCCGTTGGTTGTTTGCGT
GCCATAAGTACAATCCTTGAATCTGTTAGCAGTCTCCCGCAGCTGTTTGTTGAGATAGAA
CCAACTATACTTCCAATAATGCAGAAAATGTTGACCACTGATGGCCAAGGTATACTCATA
TAGTTTTGGGATATAGTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11