Sequences from confirmation amplicon


Confirmation sequences for line 122F04 and hit At2g45910 are shown.
Line ID 122F04
Locus At2g45910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18894265
Primers used for PCR DE05 - 8474, show primer details
   
Sequence Name 47-K019240-022-122-F04C-8409
GenBank Accession LN506049 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K019240-022-122-F04C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAAGCCATGTAGGGTTTCTTCCTCTTCTTCT
TCTTCCTTATCCGTAGACTCTAGGGTTTCAATACGATTCGAGTGTGTCTGTTTGCTCTCT
AGTCAAAAATTTCACTCTGGTTAGAAACTTTCCCTTTTGGATTGTGAAACTGCTATTTTA
AATCACTGCTTATTGATTCGTGTTCCGGAGTGATCTTGGTGAATATTCTTAATTTGTTTC
GTTGTTTTGTGGTTTTAGATATCAAACGATCTGGTTTGTCGGATTTGATATTGGAGCAAT
GGCTTTGGTGAGTCCAATTCCAGCAATGGGTGAAAGAGCTGGATCCATGAGATTTCACGG
AATTGGTAGCCCAGGATCAAGGTCTAGTAGGAGCGGGATTATGGACGAACCTGTCTCGCG
GCTGATTGACGAGAAGATCTTCGTTGCAGTGGATAAACACGTGGCCAAGAGCAAATCTAC
ACTCATTTGGGCTTTGCAAAACACTGGAGGCAAGAAGATTTGCCTTATTCATGTTCATCA
GCCCTCCCAAATGATCCCACTTAGTATGTTCCCTTACACCTTGTCTCTGTGCTTTTTTTC
CACTTAGTCTTG
   
Sequence Name 48-K019240-022-122-F04C-DE05
GenBank Accession LN506374 [GenBank]
Sequencing primer gene specific primer, DE05: 5'-GTGACCACAAACAAGACTAAGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K019240-022-122-F04C-DE05
AAACACAGAGACAAGGTGTAAGGGAACATACTAAGTGGGATCATTTGGGAGGGCTGATGA
ACATGAATAAGGCAAATCTTCTTGCCTCCAGTGTTTTGCAAAGCCCAAATGAGTGTAGAT
TTGCTC

Last Updated on 06.12.2018 16:11