Sequences from confirmation amplicon


Confirmation sequences for line 123D06 and hit At5g04150 are shown.
Line ID 123D06
Locus At5g04150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1138949
Primers used for PCR H101 - 8474, show primer details
   
Sequence Name 47-K014834-022-123-D06C-8409
GenBank Accession LN506026 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K014834-022-123-D06C-8409
AGCTGATAAACAAAGCAGTGATGGACTCAATAGATTCTTCTTCCTCTCAACGGATCGCAG
CAAATTGGCTCACTGACACAGAGATTGCTGTCCAGATTGCTACGTCGAAATGGACATCTG
TTTCAGACATGTTGCTTAGGTTAGAACAAAACGGGCTTAATGTCATAAGCGTCTCTTCTT
CCGTTTCTTCCACCGCAAGGATCTTCTACACTCTACATCTTCAGGTACTTGTATAAAGAT
ATCAGAAGGTCATGTTCTTCTTACATGGAACTCATTAAGAGAAACTAAAATCTATTGTTT
TATTCTTCTTTTTGTGCAGATGAGAGGAGATTGCAAAGTGAGACTGGAGGAACTCATCAA
TGGTATGCTTTTGCGGNAAATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAA
AAAAAAAAAGAGTAATCCCCAATTTTTTTTCAATTCAAAAATGTAAATGCCCGCAGCGTT
ATTATAAAATGAAAGTACTTTTTGATAAAACGACAAATTACGACCCGGCGTATTTATAGG
CGAAAGCAATAAACAAATTTTTCTAATTGGGAAATTT
   
Sequence Name 48-K014834-022-123-D06C-H101
GenBank Accession LN506353 [GenBank]
Sequencing primer gene specific primer, H101: 5'-CAAGAGCATACCATTGATGAGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K014834-022-123-D06C-H101
CCAGTCTCACTTTGCAATCTCCTCTCATCTGCACAAAAAGAAGAATAAAACAATAGATTT
TAGTTTCTCTTAATGAGTTCCATGTAAGAAGAACATGACCTTCTGATATCTTTAGACAAG
TACCTGAAGATGTAGAGTGTAGAAGATCCTTGCGGTGGAAGAAACGGAAGAAGAGACGCT
TATGACATTAAGCCCGTTTTCTTCTAACCTAAGCAACATGTCTGAAACAGATGTCCATTT
CGACGTAGCAATCTGGACAGCAATCTCTGTGTCAGTGAGCCAATTTGCTGCGATCCGTTG
AGAGGAAGAAGAATCTATTGAGTCCATCATTGCTTTGTTTCTCAGCTGCTCTTGGTGAGT
CCATGATCCAATTATTCAATTGTATATGATCCGTCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCA
AATGGGGGCTTAGATGAGAAACTTCACGATCGATGCCTTGAT

Last Updated on 06.12.2018 16:11