Sequences from confirmation amplicon


Confirmation sequences for line 125A08 and hit At4g30020 are shown.
Line ID 125A08
Locus At4g30020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14680260
Primers used for PCR CR12 - 8474, show primer details
   
Sequence Name 21-K017118-022-125-A08C-CR12
GenBank Accession LN495452 [GenBank]
Sequencing primer gene specific primer, CR12: 5'-CAGACCATATTAAAGAGCCTGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K017118-022-125-A08C-CR12
CAAGAATATCTGGTTTGAGAAGATCAGCATCCTGAAAGCTGAAATCTTTGGTATTGGGTC
CTCGAGCTGAGAACAGAGCAACTTCAGGTGCGGATTTGTGAAGAATGGGTTCCAAACCGT
CTCCGATGCTTCCTTCAGCTTTAAAGTCCTTTACCCTTCCCATCCAATCTCTTGACGTTG
TGACATTGTAGTAATCAATCAAATCCTAGAGAAGAAAAAATGGCATTCCAAGTTAAGGAA
AAGACGAGGGAGGAAATAACTGTATAGTGAACGCAACAAAACTATATTTTACCATTGACT
TAGAGACATCTGTAATCAGAATCCCAGGAATGCAAGAAGGAACGGGATCGAATTTTGTTC
CTGGAGAAACGTTTTCGACAACAAGAACGAAACCAGCAGCGCCTAGATGCTTGGCAGTTT
CAGCAACTTTCTTGATGGAAGCTGAACCAGCAACGAAATTGAAAGAATATCCACAAAGCA
GAATGTTTCCTTCGACTAATTTTTTGTTCAAGACTTCTGGCTTCTGGCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACTTTTTGATAAAACGACAAA
   
Sequence Name 22-K017118-022-125-A08C-8409
GenBank Accession LN495897 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K017118-022-125-A08C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCCAGAAGCCAGAAGTC
TTGAACAAAAAATTAGTCGAAGGAAACATTCTGCTTTGTGGATATTCTTTCAATTTCGTT
GCTGGTTCAGCTTCCATCAAGAAAGTTGCTGAAACTGCCAAGCATCTAGGCGCTGCTGGT
TTCGTTCTTGTTGTCGAAAACGTTTCTCCAGGAACAAAATTCGATCCCGTTCCTTCTTGC
ATTCCTGGGATTCTGATTACAGATGTCTCTAAGTCAATGGTAAAATATAGTTTTGTTGCG
TTCACTATACAGTTATTTCCTCCCTCGTCTTTTCCTTAACTTGGAATGCCATTTTTTCTT
CTCTAGGATTTGATTGATTACTACAATGTCACAACGTCAAGAGATTGGATGGGAAGGGTA
AAGGACTTTAAAGCTGAAGGAAGCATCGGAGACGGTTTGGAACCCATTCTTCACAAATCC
GCACCTGAAGTTGCTCTGTTCTCAGCTCGAGGACCCAATACCAAAGATTTCAGCTTTCAG
GATGCTGATCTTCTCAAACCAGATATTCTTGCTCCAGGCTCT

Last Updated on 06.12.2018 16:11