Sequences from confirmation amplicon


Confirmation sequences for line 125E08 and hit At3g50850 are shown.
Line ID 125E08
Locus At3g50850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18901724
Primers used for PCR AY52 - 8474, show primer details
   
Sequence Name 01-K025400-022-125-E08C-8409
GenBank Accession LN486423 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K025400-022-125-E08C-8409
GAAGCCATTTCTCGAACTTAGCCACGACTTGAGCGTTTGCGTCGGCGTTGAATTTGAGAT
TCTCGATTACGTTTGGGAGATCCGTCACCGTGACATTAGCACCTAGTGTCGCAGCCGCTG
CGATACCGACGATTCCCGTTCCAGATCCGAGCTCGACGATGTTAATCGGAGAAGAAGAAT
CTGAACCGTCGGATTGAAAACTCGAGAAGGTGCGATTGAGAGGACTAATGTTTGGGTCAC
GACGGTAGTTATCTAAAAGCGTGACGAGAGTTGTTGCAGGTATCCAGAGCTTGAAGGCAA
TGCCTTGTGTCGGTAACTGACGAATCACAACCGTTGATTCGATCGAATTGATCCGGTACT
TCTGAAGCTCCGATTCTACGTCGACTACTTT
   
Sequence Name 02-K025400-022-125-E08C-AY52
GenBank Accession LN486879 [GenBank]
Sequencing primer gene specific primer, AY52: 5'-AAAAGTAGTCGACGTAGAATCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K025400-022-125-E08C-AY52
TTCGATCGAATCAACGGGTGTGATTCGTCAGTTACCGACACAAGGCATTGCCTTCAAGCT
CTGGATACCTGCAACAACTCTCGTCACGCTTTTAGATAACTACCGTCGTGACCCAAACAT
TAGTCCTCTCAATCGCACCTTCTCGAGTTTTCAATCCGACGGTTCAGATTCTTCTTCTCC
GATTAACATCGTCGAGCTCGGATCTGGAACGGGAATCGTCGGTATCGCAGCGGCTGCGAC
ACTAGGTGCTAATGTCACGGTGACGGATCTCCCAAACGTAATCGAGAATCTCAAATTCAA
CGCCGACGCAAACGCTCAAGTCGTGGCTAAGTTCGAGAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11