Sequences from confirmation amplicon


Confirmation sequences for line 125F10 and hit At1g13040 are shown.
Line ID 125F10
Locus At1g13040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4447540
Primers used for PCR 14fb - 8474, show primer details
   
Sequence Name 38-K110825-0022-125-F10C-14fb
GenBank Accession LN503019 [GenBank]
Sequencing primer gene specific primer, 14fb: 5'-TCTGAAATTAGGGCACAAAGAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K110825-0022-125-F10C-14fb
GCTTCACAGAGGCTAAATGTGTGAAAGATATGATCAGAAAACTCTGATATAGTTCGGTGA
CATTTACGATTTAGAACAACCCAGCTCCGTGTTAAACCTTGCTTCTTTTGATACTTACTC
AGAATCCGTCGGTCTTCAAACACCAGACGAACCCTCACCTTCTCTTCCTCCATCGTAGAC
GAACTTCAGATAAGTAAAATGAGAGAAATTTCACTCAAGTTTTGAGCTTTCGGAGAAAAT
CCTTAAACCCCTGTTGAGCTCCAAAGACGGAGAAGAAGGAGAAGGGGAGAAACTGAAAGC
CAAACCTAACACTCTCGTTCACAGAGAGGTTTTAGTAAAATATGATTGGGCCTTATTTAA
AGCCCATTTAAGCCCATATCAAGAAAGCCTCAGATTAATTAATGTTGGAGCCTTCGTCTT
CTTTTTTTTTTTTTTCCCCCCNGCTAAAGCCAAAAAACAGCCCTCCTCCTCAATTTTAAT
TCCCCCCATTGAAAAAAAAAAAAGGCCCCGGGTATTTTTGAAAAAAAAAGGGTTTCCTGT
CCGGGAAATTCTCCGGGGTCCCCAAGGGGTTTGGGGGGCCATTGGGGAAAAAAAAAAGAG
TTTTTCCCCTTTTTTTTTCCTTTCAAAAAGGGAAAATTCCC
   
Sequence Name 22-K110824-0022-125-F10C-8409
GenBank Accession LN496163 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K110824-0022-125-F10C-8409
CTTTCTTTGTCATCATCACCGTTGCCTCTTCTTTGTTTCAATGCGGCGACATCAGATCGA
CGACGACGGCTGGTCTTGAGCTTAAGCTAGAGGGAAAAAAAAAAAAAAAAAAAACAAGGC
CCCCACCTTAATTAATCCGGGGGTTTTTTGAATGGGGCTTAAAGGGGCTTTAAAAAAGGC
CCAATCCTTTTTTACCAAAACCCCCTTTGAAAAAAAAAGTTTAGGTTTGGTTTTCAATTT
CCCCCCTTCCCCTTTTTCCCCCTTTTTGGAACCCAAAGGGGGTTTAAGGATTTTCCCCCA
AAGCCCAAAACTTGGGGAAAATTTCCCCCCTTTTACTTTTCCAAAATTCCCCCCCCAAGG
AAAAAAAAAAGGGGAGGGTTCCTCCGGGTTTTAAAAACCCACGGATTTCGAATAAATTTC
AAAAAAAACCAGGTTTAACCCGGGACTGGGTTTTTCCAAATCCAAAATGGCCCCCAAATT
ATTCAAAATTTTCCGAACAATTTTTTCCACACTTTACCCCCTTGAAAAGGGGGCCCCCGG
GCCCCCCCCTATTGGGACGTTAACCCTTTTTGGGCCCCAAATTTCAAAA

Last Updated on 06.12.2018 16:11