Sequences from confirmation amplicon


Confirmation sequences for line 130F06 and hit At5g10570 are shown.
Line ID 130F06
Locus At5g10570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3342600
Primers used for PCR H61a - 8474, show primer details
   
Sequence Name 41-K014709-022-130-F06C-8409
GenBank Accession LN503884 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K014709-022-130-F06C-8409
TCCGTTTTTTTGTACTAACCGTTGATACAACCAAACCCGGTTTCGTAGGACAACATATAT
CAATATGCGTATTAACTTCCCTTTGATCTACTTCAAACTGCAAAAGATTCGTTTTCGTTA
TAGCCAGAAAAATTGTCTGCACCTTCAAGTGATTATAATTTTTTGAGTGATATCTAATGG
GAATCAAATTGATTCAAGTATATGATGAGGGTGTTTGTAACAAATGATTTGTAATGGGAT
GAATTTGTAAAAACACAAGACTTATGAGTACTGAAGCACAAATACCATAATAACATTATG
TTTTGTTTTTAGCTTTTACCTTTAGGGAGTTTCTGACCATAGATTCGTTTGTAATGAGGG
TGCTTAGATGAGAGTTGCTTCCAAGTTCTTGTTCGTCTTCTTGCAACTTGTTAATCTTGT
CCAAAAGCTCCTTCATGTAATCTATTGCATCTCCTAGTATCGATGTCCTATCCATCTGAT
TTTATTATATAATAATCATCAAAAATTATATACATTTTCTCATACAACAAACTCTATGCA
GATTCACAAAAAGCAATAACCTTTTATCAGTGAACAGTGT
   
Sequence Name 42-K014709-022-130-F06C-H61a
GenBank Accession LN504275 [GenBank]
Sequencing primer gene specific primer, H61a: 5'-CTCCGATCCATTGTCCCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K014709-022-130-F06C-H61a
CCAAGTCAAATTCCTAAACCTCATCCTTGTCACATGTTATATGGTTTTTATTTTTAAGTC
AAGAAGAAATATTCTTCCTTTTTTGTTAAGTTCTTAACGTTACATGTATGTTTTCTTCTT
GTTGAAATCTTTTTTTTGGTACACTGTTCACTGATAAAAGGTTATTGCTTTTTGTGAATC
TGCATAGAGTTTGTTGTATGAGAAAATGTATATAATTTTTGATGATTATTATATAATAAA
ATCAGATGGATAGGACATCGATACTAGGAGATGCAATAGATTACATGAAGGAGCTTTTGG
ACAAGATTAACAAGTTGCAAGAAGACGAACAAGAACTTGGAAGCAACTCTCATCTAAGCA
CCCTCATTACAAACGAATCTATGGTCAGAAACTCCCTAAAGGTAAAAGCTAAAAACAAAA
CATAATGTTATTATGGTATTTGTGCTTCAGTACTCATAAGTCTTGTGTTTTTACAAATTC
ATCCCATTACAAATCATTTGTTACAAACACCCTCATCATATACTTGAATCAATTTGATTC
CCATTAGATATCACTCAAAAAATTATAATCACTTGAAGGTGCAGACAATTTTTCTGGCTA
TAACGAAAACGAATCTTTTGCAGTTTGAAGTAGATCAAAGGGAAGTTAATACGCATATTG
ATATAT

Last Updated on 06.12.2018 16:11