Sequences from confirmation amplicon


Confirmation sequences for line 133C08 and hit At3g56940 are shown.
Line ID 133C08
Locus At3g56940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21076856
Primers used for PCR PJ01 - 8474, show primer details
   
Sequence Name 05-K026133-022-133-C08C-8409
GenBank Accession LN488202 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K026133-022-133-C08C-8409
ATTTCCCGGACATGAAGCCATTTACAACATGGGATCAGAACCTTAACGAAGCAAAGTTCG
AGGCTCTGCTTCAAGAGTTCAAGACCGATTACAACCAGACACATTTCGTGAGGAACAAGG
AGTTTAAAGAAGCTGCAGACAAATTGCAAGGACCTCTCCGACAGATCTTCGTTGAGTTCC
TTGAGCGGTCTTGTACTGCTGAGTTCTCTGGTTTCCTTCTCTACAAGGAGCTTGGTCGAA
GACTCAAGGTGAGAATTTAAGAATATATATGGCTTTAAGTAATAGTCAAGTCTCAATTGT
TATGAGTTTTCAAGTGTGGTTGTTGGTTTGATGTTCCTTGTCTACGTTATGCTTGAAGCT
ATGCTCATTAGGTGGAAGTTAGAGCACATGTGAGACTTGTTGTTGTTTCTTTGGATCATT
TTATTGGCTTGATTTTGAATCTTTGATCTGATGGCATTGCAGAAAACAAACCCTGTTGTG
GCTGAGATCTTCTCTCTTATGTCTAGAGATGAAGCAAGACATGCCGGGTAAGATCTCTTA
ACACTAAGGACCTCTCTTTTTAATCTAAGTGTTTCAATTTTGATGATAAACTTTTTTTGG
CAGGTTCTTGAACAAGGGATTGTCT
   
Sequence Name 06-K026133-022-133-C08C-PJ01
GenBank Accession LN488652 [GenBank]
Sequencing primer gene specific primer, PJ01: 5'-AGAGCCAAGTTGAAATCAGACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K026133-022-133-C08C-PJ01
CCCTTGTTCAAGAACCTGCCAAAAAAAGTTTATCATCAAAATTGAAACACTTAGATTAAA
AAGAGAGGTCCTTAGTGTTAAGAGATCTTACCCGGCATGTCTTGCTTCATCTCTAGACAT
AAGAGAGAAGATCTCAGCCACAACAGGGTTTGTTTTCTGCAATGCCATCAGATCAAAGAT
TCAAAATCAAGCCAATAAAATGATCCAAAGAAACAACAACAAGTCTCACATGTGCTCTAA
CTTCCACCTAATGAGCATAGCTTCAAGCATAACGTAGACAAGGAACATCAAACCAACAAC
CACACTTGAAAACTCATAACAATTGAGACTTGACTATTACTTAAAGCCATATATATTCTT
AAATTCTCACCTTGAGTCTTCGACCAAGCTCCTTGTAGAGAAGGAAACCAGAGAACTCAG
CAGTACAAGACCGCTCAAGGAACTCAACGAAGATCTGTCGGAGAGGTCCTTGCAATTTGT
CTGCAGCTTCTTTAAACTCCTTGTTCCTCACGAAATGTGTCTGGTTGTAATCGGTCTTGA
ACTCTTGAAGCAGAGCCTCGAACTCTGCTTCGTTAAGGTTCTGATCCCATGTTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATNGGTCAATTATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATT

Last Updated on 06.12.2018 16:11