Sequences from confirmation amplicon


Confirmation sequences for line 135B06 and hit At4g37250 are shown.
Line ID 135B06
Locus At4g37250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17528535
Primers used for PCR BW05 - 8474, show primer details
   
Sequence Name 02-K021167-022-135-B06C-8409
GenBank Accession LN486848 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K021167-022-135-B06C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAGTCTCTTACACATGTGTGTATATGAGTT
TGCTGAATTTTGTGTGTCCTTGGGCAGGAAAAGGAGGAGGGTCTTCTTCACCGTATCACC
TACCGTGGGAAACTCGGCTCAAGATAGCAAAGGGCATTGCACGTGGGCTGGCTTATCTCC
ACGAGAAGAAGCATGTGCATGGAAACTTGAAGCCTAGCAACATACTTTTGGGCCATGACA
TGGAACCCAAAATAGGCGATTTCGGACTCGAAAGGCTTCTTACAGGCGAAACAAGCTACA
TTCGAGCTGGTGGATCTTCTAGGATTTTCAGTAGTAAGCGTTACACAACTTCCTCGCGAG
AATTCTCGTCTATCGGGCCTACACCAAGCCCGAGTCCGAGCTCGGTGGGGGCGATGTCAC
CCTACTGCGCACCAGAGTCTTTCCGAAGCCTAAAACCGAGTCCGAAATGGGACGTTTACG
GCTTTGGAGTGATCCTTCTCGAGCTTCTCACGGGAAAGATTGTGTCTGTTGAAGAGATTG
TGCTAGGAAACGGACTT
   
Sequence Name 06-K021167-022-135-B06C-BW05
GenBank Accession LN488613 [GenBank]
Sequencing primer gene specific primer, BW05: 5'-AGACGCACAGCTATAGCCTAACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K021167-022-135-B06C-BW05
AATCGAGTAAAAACTCTTGTTTGCCATCTAACTCGCCGCGGATCGCCACATCAGCCATTC
TCACTGCGCGGTGCCCGTCCTCAACCGTAAGTCCGTTTCCTAGCACAATCTCTTCAACAG
ACACAATCTTTCCCGTGAGAAGCTCGAGAAGGATCACTCCAAAGCCGTAAACGTCCCATT
TCGGACTCGGTTTTAGGCTTCGGAAAGACTCTGGTGCGCAGTAGGGTGACATCGCCCCCA
CCGAGCTCGGACTCGGGCTTGGTGTAGGCCCGATAGACGAGAATTCTCGCGAGGAAGTTG
TGTAACGCTTACTACTGAAAATCCTAGAAGATCCACCAGCTCGAATGTAGCTTGTTTCGC
CTGTAAGAAGCCTTTCGAGTCCGAAATCGCCTATTTTGGGTTCCATGTCATGGCCCAAAA
GTATGTTGCTAGGCTTCAAGTTTCCATGCACATGCTTCTTCTCGTGGAGATAAGCCAGCC
CACGTGCAATGCCCTTTGCTATCTTGAGCCGAGTTTCCCACGGTAGGTGATACGGTGAAG
AAGACCCTCCTCCTTTTCCTGCCCAAGGACACACAAAATTCAGCAAACTCATATACACAC
ATGTGTAAGAGACTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAA

Last Updated on 06.12.2018 16:11