Sequences from confirmation amplicon


Confirmation sequences for line 135D07 and hit At3g51260 are shown.
Line ID 135D07
Locus At3g51260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19031074
Primers used for PCR CK07 - 8474, show primer details
   
Sequence Name 24-K017715-0022-135-D07C-8409
GenBank Accession LN496797 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K017715-0022-135-D07C-8409
AGATTTCCCGGACATGAAGCCATTTACATCCGTGTAGAGTTTTTGTATTCTCCCCCGATG
GTCACCTCTTCCAAGTAGAGTATGCCCTAGAAGCCGTCCGAAAGGGTAACGCCGCCGTCG
GAGTCCGCGGCACAGATACTGTCGTCCTCGCCGTCGAAAAGAAATCCACCCCTAAGCTCC
AGGATTCCAGGTATTGCTCCGATCTCTACCGATACAAGATGCTTAAACCGGATTTAGTCC
ATTTACTTTTGTTTCTGCACTTTGATTTTGGTTTTAAGCTTCACCTGGATAGCTCGCTTG
ATTTACTCAATTGCTACTTGTATGATCACTGTTTCAATCCTTCTGAACTAAATCCTGATG
TTGGGATTTTGTATCACTTCGTAATTGCGAATCTAGTATTGTTAGATCGTAGAATCTGAG
CTGAACCCTAGTTTAAGTCTTTTTTCTCTACGAGGGTACAATTACAGTTCCTGTATCTCA
AACGTGTTTTTTTGTATAAAAACACGTTTTTGGTTATACTCTGCAAA
   
Sequence Name 23-K017715-0022-135-D07C-CK07
GenBank Accession LN496355 [GenBank]
Sequencing primer gene specific primer, CK07: 5'-AGATCTATTGGAGCAAGAGCTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K017715-0022-135-D07C-CK07
AGATACGAAGACGATATTGCTTAGATTTCAGCAAGTTCAACACCAAAATTATATTTTTAC
GCAGTGAATGATCAATTTGCAGAGTATAACCAAAAACGTGTTCTTATACAAAAAAACACG
TCTGAGATACAGGAACTGTAATTGTACACTCGTAGAGAAAAAGACTTAAACTAGGGTTCA
GCTCAGATTCTACGATCTAACAATACTAGATTCGCAATTACGAAGTGATACAAAATCCCA
ACATCAGGATTTAGTTCAGAAGGATTGAAACAGTGATCATACAAGTAGCAATTGAGTAAA
TCAAGCGAGCTATCCAGGTGAAGCTTAAAACCAAAATCAAAGTGCAGAAACAAAAGTAAA
TGGACTAAATCCGGTTTAAGCATCTTGTATCGGTAGAGATCGGAGCAATACCTGGAATCC
TGGAGCTTTAGGGTGGATTTCTTTTCGACGGCGAGGACGACAGTATCTGTGCCGCGGAC
Primers used for PCR 01ex - 8474, show primer details
   
Sequence Name 19-K123356-0022-135-D07C-01ex
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 01ex: 5'-GTGACTTATCCTGCCTTCGTTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K123356-0022-135-D07C-01ex
TTGCACCATATGTTATGTATTCTACCAGATTAATTTGACAAAAAACAATGTTAGGAGTTT
TAATGTTTTGAAGAAGTGGGCCAGATGTTTGGAGTTTTAGGCCAATGTAGCTCATCTTGG
GCCCAGTACAGAAATAAATCCAGTGATGATTCTTTCTGATTGGCTGGAAGGTGTGTGTAC
GTGAACCTGTCCGCAGGTGATTAAAACAGAGCTGGGCTTTTGGCTTGTCGCCTAAGTCGG
GAATCGTTTACTGTGAAATAAAAACGAGAGAGAGTTTTTGAAGAAGAAGAAAGAACACTC
ACAAGATCCAGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCATATATATTAT
   
Sequence Name 02-K123355-0022-135-D07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K123355-0022-135-D07C-8409
TTACATTGAATATATTCTGGATCTTGTGAGTGTTCTTTCTTCTTCTTCAAAAACTCTCTC
TCGTTTTTATTTCACAGTAAACGATTCCCGACTTAGGCGACAAGCCAAAAGCCCAGCTCT
GTTTTAATCACCTGCGGACAGGTTCACGTACACACACCTTCCAGCCAATCAGAAAGAATC
ATCACTGGATTTATTTCTGTACTGGGCCCAAGATGAGCTACATTGGCCTAAAACTCCAAA
CATCTGGCCCACTTCTTCAAAACATTAAAACTCCTAACATTGTTTTTTGTCAAATTAATC
TGGTAGAATACATAACATAGGTGCAATTAGTGTTTCTAAGCAAACACTAAAAAAGGTAAA
CGAAGGCAGGATAAGTCA

Last Updated on 06.12.2018 16:11