Sequences from confirmation amplicon


Confirmation sequences for line 135H09 and hit At5g41760 are shown.
Line ID 135H09
Locus At5g41760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16706924
Primers used for PCR MJ32 - 8474, show primer details
   
Sequence Name 06-K029908-022-135-H09C-8409
GenBank Accession LN488680 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K029908-022-135-H09C-8409
AAATGAGAGAGCTCCTGTGAAGTGACGAAGAGACGAAGAAGAAAGAGAACATTAATGGCG
GCTACTCCGTGGTACTTTGTCGCAGTACTTCTCACCATTCTTACCAGCTCTCAGGTTCCA
ATTTCCAACCCTAATTCTCCAATTCCTTTCTGTACTTCTCTTGCTAATTATGATTGAATC
GAGATTCGTTTCCTCTATGTTTCGACATTTCCTGAACTCTTAGAATTTCGGCGACTTTAT
AGATCTAGAAATTGAGTTATCGTTGGTGTTATTGTCGCCAAATCTAGTTCTGGATCATGT
ATGTCGGTTAGAGTGTGATAATTGCTATTTTCCACGGCTTCGAACAATGTCATGTTAGAT
CGGTAGATTCGATTATTCCAGTTGATTCTGCTGTTAATTTGCGAGTGCTTGATTGATTTG
ATTTTTCGATATTATATGTAGCTGAAGAAGTTTAGTGAGAATTTGTTTTCACCAAAAGCT
GTTATTAGTCTAACACAAGTCATGATCATCATTCTTTATGTTCTTCTGGCTTTAGGTTTT
AGATAGAAACTGAACATAATTCACTGGATAGATTCGAAAATGAGAGAAGTTTTTGTTT
   
Sequence Name 06-K029908-022-135-H09C-MJ32
GenBank Accession LN488681 [GenBank]
Sequencing primer gene specific primer, MJ32: 5'-CACAGTGAGAGAATGGAAACAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K029908-022-135-H09C-MJ32
ATTATGTTCAGTTTCTATCTAAAACCTAAAGCCAGAAGAACATAAAGAATGATGATCATG
ACTTGTGTTAGACTAATAACAGCTTTTGGTGAAAACAAATTCTCACTAAACTTCTTCAGC
TACATATAATATCGAAAAATCAAATCAATCAAGCACTCGCAAATTAACAGCAGAATCAAC
TGGAATAATCGAATCTACCGATCTAACATGACATTGTTCGAAGCCGTGGAAAATAGCAAT
TATCACACTCTAACCGACATACATGATCCAGAACTAGATTTGGCGACAATAACACCAACG
ATAACTCAATTTCTAGATCTATAAAGTCGCCGAAATTCTAAGAGTTCAGGAAATGTCGAA
ACATAGAGGAAACGAATCTCGATTCAATCATAATTAGCAAGAGAAGTACAGAAAGGAATT
GGAGAATTAGGGTTGGAAATTGGAACCTGAGAGCTGGTAAGAATGGTGAGAAGTACTGCG
ACAAAGTACCACGGAGTAGCCGCCATTAATGTTCTCTTTCTTCTTCGTCTCTTCGTCACT
TCACAGGAGCTCTCTCATTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATNTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11