Sequences from confirmation amplicon


Confirmation sequences for line 136D11 and hit At5g11300 are shown.
Line ID 136D11
Locus At5g11300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3602383
Primers used for PCR AS03 - 8474, show primer details
   
Sequence Name 20-K018323-022-136-D11C-AS03
GenBank Accession LN495009 [GenBank]
Sequencing primer gene specific primer, AS03: 5'-CGCCACACAATAGCTACAGAATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K018323-022-136-D11C-AS03
CAGACAAAACTGGCAAAGAGACGACATAACATCCAATGTCTGAAAATAGGTATTTTGATC
ACCTGACTTGGGCTCTGTTTTCGATGTTGCTTGGTGTCATCTTGAGAATAGTGATGTGAC
TCGTTTGGGAGATGTCAGCTTTGCCACGCTCTTAAACTATCAAAGTCATACAGAATTAAA
ACCAGTTCAATCATTCATTCACTTTTCTTCAACAATCAGTAGTTTGATTTAACGGTGTTT
TGTAGAGAAATGTACCTTTGGTTGGTTGTATTTCTCACGGGTGGCAGCGAGAGTACAGCC
ACTGGTGTTGAGCTGCAAGTCCTCCATGGCGAGAACTGTGTTCTTCAGCTCAGCTACCTC
ATATCTGGTGTAGTGTTGCAGAGTAGGGTTCTATAACCAAACATCATTCTTTTTATCAGC
ACAAAAAAGAGGCAAACACAAAAACAACCTTTTTCAAGATTCAAGAAACATGAGCTGACC
CAAGGATGGTCAGTTTGGTCGAGTGTCCATCGGGCTAGGAAAACAGCTGAAGCAGCAATT
AGTGATGGCAGGAACCTTAGGAAACTATATTCCACCAGTGTCAATTCGGCGAGATAGTTT
GCTAAATACTCCAGTTCAATGAAAGGCACCTGCAAAATAATTATAAGAAACAACGCACTA
CATTCAGATCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATT
   
Sequence Name 24-K018323-022-136-D11C-8409
GenBank Accession LN496800 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K018323-022-136-D11C-8409
CCATGTAGATTTCCCGGACATGAAGATCTGAATGTAGTGCGTTGTTTCTTATAATTATTT
TGCAGGTGCCTTTCATTGAACTGGAGTATTTAGCAAACTATCTCGCCGAATTGACACTGG
TGGAATATAGTTTCCTAAGGTTCCTGCCATCACTAATTGCTGCTTCAGCTGTTTTCCTAG
CCCGATGGACACTCGACCAAACTGACCATCCTTGGGTCAGCTCATGTTTCTTGAATCTTG
AAAAAGGTTGTTTTTGTGTTTGCCTCTTTTTTGTGCTGATAAAAAGAATGATGTTTGGTT
ATAGAACCCTACTCTGCAACACTACACCAGATATGAGGTAGCTGAGCTGAAGAACACAGT
TCTCGCCATGGAGGACTTGCAGCTCAACACCAGTGGCTGTACTCTCGCTGCCACCCGTGA
GAAATACAACCAACCAAAGGTACATTTCTCTACAAAACACCGTTAAATCAAACTACTGAT
TGTTGAAGAAAAGTGAATGAATGATTGAACTGGTTTTAATTCTGTATGACTTTGATAGTT
TAAGAGCGTGGCAAAGCTGACATCTCCCAAACGAGTCACATCACTATTCTCAAGATGACA
CCAAGCAACATCGAAAACAGAGCCCAAGTCAGGTGATCAAAATACCTATTTTCAGACATT
GGATGTTATGTCGTCTCTTTGCCAGTTTTGTCTGTCTGTAATTCT

Last Updated on 06.12.2018 16:11