Sequences from confirmation amplicon


Confirmation sequences for line 136E12 and hit At5g42520 are shown.
Line ID 136E12
Locus At5g42520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17001252
Primers used for PCR KD75 - 8474, show primer details
   
Sequence Name 53-K103246-0022-136-E12C-KD75
GenBank Accession LN508121 [GenBank]
Sequencing primer gene specific primer, KD75: 5'-TGTGTTTTGTGTTTTCCCAAATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K103246-0022-136-E12C-KD75
TATCGATGATACTGTGTTTCTTTTAGCTGAGTTTGATATCTCACAGAGTTTATGTGTATT
GGTTAGAATCGATTGATTGATGTGCAAATCCTGAAACAAAATGGTACCATTTCGATATTA
ATGTTGCTATTTTTAGTCATCTGAGTTTGAGTGTTTTTGTTGAAAGTTTTTCAGAAAATA
CTCATAGATGGACAAACAATTGTTTGCTTTGTCTCTGCTGAATCATATTCTGGAACTCAA
GTATAGATTTTTTTTTTGCTTCAAAGTCGTTTATGTATGTTCACTAATACTAGCAATGCT
TTGGTGATGGGCTATTTTGCTTCAAAGTAGTTTATTTATGAAATGGTCTTTTTTGACACT
GTTTAAGGATGGGCTATTTGCAGGAGGATTAAAAGATGGACCAGTCTTTTATTTTGATAT
TCCGTTAGCTTGTTCAGTTATGGTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACTTGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 05-K103247-0022-136-E12C-8409
GenBank Accession LN488314 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K103247-0022-136-E12C-8409
GCCATTTACATTGAATATATCCTGAACAATAACTGAACAAGCTAACGGAATATCAAAATA
AAAGACTGGTCCATCTTTTAATCCTCCTGCACATAGCCAATCATTAAACAGTGTCAAAAA
AGACCATTTCATAAATAAACTACTATGAAGCAAAATAGCCAATCACCAAAGCATTGCTAG
TATTAGTGAACATACATAAACGACTATGAAGCAAAAAAAAAATCTATACTTGAGTTCCAG
AATATGATTCAGCAGAGACAAAGCAAACAATTGTTTGTCCATCTATGAGTATTTTCTGAA
AAACTTTCAACAAAAACACTCAAACTCAGATGACTAAAAATAGCAACATTAATATCGAAA
TGGTACCATTTTGTTTCAGGATTTGCACATCAATCAATCGATTCTAACCAATACACATAA
ACTCTGTGAGATATCAAACTCAGCTAAAAGAAACACAGTATCATCGATTATAGTCATAGT
GATAATCATCAAAAGCTAATTTGGGAAAACACAAAA

Last Updated on 06.12.2018 16:11