Sequences from confirmation amplicon


Confirmation sequences for line 136G06 and hit At2g22770 are shown.
Line ID 136G06
Locus At2g22770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9684791
Primers used for PCR H20a - 8474, show primer details
   
Sequence Name 05-K014711-022-136-G06C-8409
GenBank Accession LN488136 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K014711-022-136-G06C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTAAATGGATGATTAGTTTT
ATTCTTTCAACATTAACTTTTTTTTACTCTCTTGTCAAAAGAGAAAAAGAGTTCTTGTAA
TGGATGATTCAAGCTTTATGGATTTGATGATCGACACTGACGAGTATCTGATCGATGACT
GGGAATCCGATTTCCCGATATGCGGGGAAACTAACACCAACCCCGGTTCCGAGTCTGGAT
CTGGAACCGGGTTTGAGTTGCTAGCAGAAAGACCAACGAAGCAAATGAAAACCAACAACA
ACATGAATTCAACATCTTCTTCTCCGTCGTCTTCTTCTTCTTCTGGTTCTCGCACGTCAC
AAGTGATCTCATTTGGGTCTCCAGACACGAAAACCAACCCGGTCGAGACATCTTTGAACT
TCTCCAACCAAGTAAGCATGGATCAGAAAGTGGGGTCGAAAAGAAAAGATTGTGTTAACA
ATGGAGGAAGAAGAGAACCACATCTATTGAAAGAACATGTTTTGGCTGAACGAAAACGTC
GACAAAAGCT
   
Sequence Name 06-K014711-022-136-G06C-H20a
GenBank Accession LN488578 [GenBank]
Sequencing primer gene specific primer, H20a: 5'-AACGCTCGTTGAGCTTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K014711-022-136-G06C-H20a
CGACGTTTTCGTTCAGCCAAAACATGTTCTTTCAATAGATGTGGTTCTCTTCTTCCTCCA
TTGTTAACACAATCTTTTCTTTTCGACCCCACTTTCTGATCCATGCTTACTTGGTTGGAG
AAGTTCAAAGATGTCTCGACCGGGTTGGTTTTCGTGTCTGGAGACCCAAATGAGATCACT
TGTGACGTGCGAGAACCAGAAGAAGAAGAAGACGACGGAGAAGAAGATGTTGAATTCATG
TTGTTGTTGGTTTTCATTTGCTTCGTTGGTCTTTCTGCTAGCAACTCAAACCCGGTTCCA
GATCCAGACTCGGAACCGGGGTTGGTGTTAGTTTCCCCGCATATCGGGAAATCGGATTCC
CAGTCATCGATCAGATACTCGTCAGTGTCGATCATCAAATCCATAAAGCTTGAATCATCC
ATTACAAGAACTCTTTTTCTCTTTTGACAAGAGAGTAAAAAAAAGTTAATGTTGAAAGAA
TAAAACTAATCATCCATTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACG

Last Updated on 06.12.2018 16:11