Sequences from confirmation amplicon


Confirmation sequences for line 137C04 and hit At4g23900 are shown.
Line ID 137C04
Locus At4g23900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr:
Primers used for PCR RZ04 - 8474, show primer details
   
Sequence Name 45-K017402-022-137-C04C-RZ04
GenBank Accession LN505362 [GenBank]
Sequencing primer gene specific primer, RZ04: 5'-ATGAGACAAAAACCGGAATATGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K017402-022-137-C04C-rz4
ACATAAATTGTGTTAGCAAGAAGTATTCTCTCACAAGAAACAAACAATTTATTTTCTTGA
TGGGAACACACGTAGGATCAAGTAAAAGCTTCGATCAAATATTTTAGATTGGAAACGATA
CAACAGATCACTGATTCAATACAATCGAGAATCTAGAAAACGAAGAAAACCACAGGTAGA
TTCATAAAATATTTGACGTCTATCTAGATGTATCATCCCATGACGTTTATCACATTTTCA
GGAAAAAAGAGGATCAGAGAGAAAATTTACGTACCGGAGAAGAAACGAGCATTCTTGGCT
GAAGAAAGAAGAGACCTGGCTGCTCTAGAAGCAGATCTGCAGATTTGGGAGCTCATTTTC
TCTGATACGATGCGTGAGGACTGAGAAGAGTGATGTGCAAACAGGGACCTCAAAAATGTG
AACCTATTGGAAGATTTTATACCAACATGTTTTGATCGGGTCGGATCAGACAAGGACTTA
AAGGCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGA
   
Sequence Name 46-K017402-022-137-C04C-8409
GenBank Accession LN505711 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K017402-022-137-C04C-8409
GACATGAAGCCATTTACAATTGAATATATCCTGAGCCTTTAAGTCCTTGTCTGATCCGAC
CCGATCAAAACATGTTGGTATAAAATCTTCCAATAGGTTCACATTTTTGAGGTCCCTGTT
TGCACATCACTCTTCTCAGTCCTCACGCATCGTATCAGAGAAAATGAGCTCCCAAATCTG
CAGATCTGCTTCTAGAGCAGCCAGGTCTCTTCTTTCTTCAGCCAAGAATGCTCGTTTCTT
CTCCGGTACGTAAATTTTCTCTCTGATCCTCTTTTTTCCTGAAAATGTGATAAACGTCAT
GGGATGATACATCTAGATAGACGTCAAATATTTTATGAATCTACCTGTGGTTTTCTTCGT
TTTCTAGATTCTCGATTGTATTGAATCAGTGATCTGTTGTATCGTTTCCAATCTAAAATA
TTTGATCGAAGCTTTTACTTGATCCTACGTGTGTTCCCATCAAGAAAATAAATTGTTTGT
TTCTTGTGAGAGAATACTTCTTGCTAACACATTTTATGTGAAACTATTGGATACACAAAT
TTAGACTCTCATATTCCGGTT

Last Updated on 06.12.2018 16:11