Sequences from confirmation amplicon


Confirmation sequences for line 137C11 and hit At5g62000 are shown.
Line ID 137C11
Locus At5g62000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24911453
Primers used for PCR AT21 - 8474, show primer details
   
Sequence Name 29-K025674-022-137-C11C-8409
GenBank Accession LN499135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K025674-022-137-C11C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGAAATTAAAGAAACCTACC
TTTAAATCTACATTAATAACTCGACAGAGAAGCTTTGACGGAAGATCATAGAGAGGCATC
TGTTGTTCTGCCGCCTGGTTCGTCGAAGCCTCCACCTAATCATAAAACAATGGAAACCGA
CAAAGTTCATTAGAAAATATCTCAACAGGTACGAATCACAGAGGGGTTAACTCAAAGATT
TAGCCAAAAATAGCAAGAACTCATAGATGAAATATCTCACCTGCTCGATGTGTCCTTGAG
GAAAATAGAAGACTCGGTCGTCTTGTCTAGGAACCGTCACAAGCGGACCAGCACAAGCGT
GCCATAGCTCTCTGTAAAGAGCAGCCTCAGGGTCCACTATGAAGACGAAGAAGAAGAAGA
TAAATTAGATCAAGTCAGTCTGTAACCAAAACCAAGCTTAAGAAACCCTAAGAAACGGAG
ACTTACAAGCACGCTCAGCCGCAGCGGATCGGGTAGAATTACTTTTTTGCCCCTCGCCGG
CGACGGAGACATTTCTAGTCTCCTTAGGGTCACTAAAACCAGAGGAGGA
   
Sequence Name 30-K025674-022-137-C11C-AT21
GenBank Accession LN499572 [GenBank]
Sequencing primer gene specific primer, AT21: 5'-GGAGATAACTTCTCCTCCTCTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K025674-022-137-C11C-AT21
CCCTAAGGAGACTAGAAATGTCTCCGTCGCCGGCGAGGGGCAAAAAAGTAATTCTACCCG
ATCCGCTGCGGCTGAGCGTGCTTGTAAGTCTCCGTTTCTTAGGGTTTCTTAAGCTTGGTT
TTGGTTACAGACTGACTTGATCTAATTTATCTTCTTCTTCTTCGTCTTCATAGTGGACCC
TGAGGCTGCTCTTTACAGAGAGCTATGGCACGCTTGTGCTGGTCCGCTTGTGACGGTTCC
TAGACAAGACGACCGAGTCTTCTATTTTCCTCAAGGACACATCGAGCAGGTGAGATATTT
CATCTATGAGTTCTTGCTATTTTTGGCTAAATCTTTGAGTTAACCCCTCTGTGATTCGTA
CCTGTTGAGATATTTTCTAATGAACTTTGTCGGTTTCCATTGTTTTATGATTAGGTGGAG
GCTTCGACGAACCAGGCGGCAGAACAACAGATGCCTCTCTATGATCTTCCGTCAAAGCTT
CTCTGTCGAGTTATTAATGTAGATTTAAAGGTAGGTTTCTTTAATTTCCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC
TA

Last Updated on 06.12.2018 16:11