Sequences from confirmation amplicon


Confirmation sequences for line 138A08 and hit At4g35830 are shown.
Line ID 138A08
Locus At4g35830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16974775
Primers used for PCR MS15 - 8474, show primer details
   
Sequence Name 52-K016395-022-138-A08C-8409
GenBank Accession LN507661 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K016395-022-138-A08C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGGTGATCCATATACCCTCAAAATTTCT
GTTCCCAGACAACACAGCGGATGCCACCAAGTCTTGTACATATATGAGAAATCTTTTAGT
TTGACCTTCGAAAATTCAAGTGAAGAAGCATTACTTCATTAACTACTTAAGGAATTACCA
TTATCAACTATTGCTGAAGCCACAGCTTCATGGATATCCCCCGAGTTTCCAATGCATGTG
GTGCACCCATAACCAACGATACTGAAGCCGAGCTGATTCAAGTACTTCTGCAAGCCACTG
CAAGACTTGAAAACTCAGGACCACACAATACAAAGGATAAGGAAATAAATAACAAAACAT
TCAACGGTGTATCTGCAGCACCTCTTTGCCAAGTACTTTGTTACAACTCCAGAGCCTGGA
GCAAGACTAGTTTTGATCCATGGCTTAACCTAATAATTGAGAAATTTCAAACACTTCTAT
TATTTCCATTGCACATAAAGATATTAAATCACTATCACACAAGTAG
   
Sequence Name 53-K016395-022-138-A08C-MS15
GenBank Accession LN507982 [GenBank]
Sequencing primer gene specific primer, MS15: 5'-CTGGAGGTTTGACTACTTGTGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K016395-022-138-A08C-ms15
TGATTAATATCTTTATGTGCAATGGAAATAATAGAAGTGTTTGAAATTTCTCAATTATTA
GGTTAAGCCATGGATCAAAACTAGTCTTGCTCCAGGCTCTGGAGTTGTAACAAAGTACTT
GGCAAAGAGGTGCTGCAGATACACCGTTGAATGTTTTGTTATTTATTTCCTTATCCTTTG
TATTGTGTGGTCCTGAGTTTTCAAGTCTTGCAGTGGCTTGCAGAAGTACTTGAATCAGCT
CGGCTTCAGTATCGTTGGTTATGGGTGCACCACATGCATTGGAAACTCGGGGGATATCCA
TGAAGCTGTGGCTTCAGCAATAGTTGATAATGGTAATTCCTTAAGTAGTTAATGAAGTAA
TGCTTCTTCACTTGAATTTTCGAAGGTCAAACTAAAAGATTTCTCATATATGTACAAGAC
TTGGTGGCATCCGCTGTGTTGTCTGGGAACAGAAATTTTGAGGGTATATGGATCACCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT

Last Updated on 06.12.2018 16:11