Sequences from confirmation amplicon


Confirmation sequences for line 138A09 and hit At3g61570 are shown.
Line ID 138A09
Locus At3g61570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22781984
Primers used for PCR 04wm - 3144, show primer details
   
Sequence Name 28-K118761-0022-138-A09C-04wm
GenBank Accession LN499058 [GenBank]
Sequencing primer gene specific primer, 04wm: 5'-GTTTGAGTCGTTTTAATTCTTGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118761-0022-138-A09C-04wm
ATCCATTTCCACCTCTGCAGGGATTGCTCCATTTCTTCTTTTCCGGAAAGCTTTCAGGCT
TCTCCTAGTAAAATATGGAAACGGCATAAGAAGGAAGGAAAAAGGTAGCTCCACATTTAA
TGAAAAGCAATGCAAAATGCGTTTATAATTAAGTTCCTTCTAGTTTAAGAACCTTGTCCA
AAGTACTAGGATGCCTAGTCAGAGACTCTGTGGCGTCCGGGAAGACTTTGCTATTGGTTA
GCTTCCTGCTTTCTTCTAAAGCAGCTTCTAGCTCACTTCGTTTCAACTATGAAGTGATGA
AAAAGCATATCAGATTCGGTTCCAGATTCCAAAAGTCAACGTAGACATACACAATGTTGG
CAATTTTTGCTTGCACAACCATTCAGCAGTATGGAACACAATAAACCTTAAGCTCGTTGT
TTTCCTTCTCCAGAGCTTTGTTAACTTCTTTCAGATGTTCCATGCCAGGTCCAATAGCAT
GGCTTTCCTGACCAGTCAACTTCATCTGCAGGTGTTTGATTTCAAGTAATTTTGCATCCA
ATTCACTACGCATTTTGCTAATCTCCATGGCTAAACGACAAACACTGATAGTTTAAACCG
AAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTT
CCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCA
   
Sequence Name 03-K118760-0022-138-A09C-3144
GenBank Accession LN487693 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118760-0022-138-A09C-3144
CAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGAT
CAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGTCGTTTAGCCATGGAGA
TTAGCAAAATGCGTAGTGAATTGGATGCAAAATTACTTGAAATCAAACACCTGCAGATGA
AGTTGACTGGTCAGGAAAGCCATGCTATTGGACCTGGCATGGAACATCTGAAAGAAGTTA
ACAAAGCTCTGGAGAAGGAAAACAACGAGCTTAAGGTTTATTGTGTTCCATACTGCTGAA
TGGTTGTGCAAGCAAAAATTGCCAACATTGTGTATGTCTACGTTGACTTTTGGAATCTGG
AACCGAATCTGATATGCTTTTTCATCACTTCATAGTTGAAACGAAGTGAGCTAGAAGCTG
CTTTAGAAGAAAGCAGGAAGCTAACCAATAGCAAAGTCTTCCCGGACGCCACAGAGTCTC
TGACTAGGCATCCTAGTACTTTGGACAAGGTTCTTAAACTAGAAGGAACTTAATTATAAA
CGCATTTTGCATTGCTTTTCATTAAATGTGGAGCTACCTTTTTCCTTCCTTCTTATGCCG
TTTCCATATTTTACTAGGAGAAGCCTGAAAGCTTTCCTGGAAAAGAAGAAATGGAGCAAT
CCCTGCAGAGGTTGGAAATGGATTTGAAGGAAACACAAAGAGAAAGACATAAGGCACGGC
AAGAATTAAACG

Last Updated on 06.12.2018 16:11