Sequences from confirmation amplicon


Confirmation sequences for line 139A12 and hit At3g23810 are shown.
Line ID 139A12
Locus At3g23810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8588186
Primers used for PCR IF01 - 8474, show primer details
   
Sequence Name 15-K019821-022-139-A12C-IF01
GenBank Accession LN492765 [GenBank]
Sequencing primer gene specific primer, IF01: 5'-CTCATGAGGGCTACTGATGTTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K019821-022-139-A12C-IF01
CTGGAAAGTTGCTGTTATCTGTGGTTATGGTGATGTAGGAAAAGGTTGTGCTGCAGCAAT
GAAAACCGCGGGTGCTCGTGTCATAGTGACTGAGATTGATCCAATCTGTGCTCTTCAGGC
TCTTATGGAAGGTCTTCAAGTTCTGACCCTTGAGGATGTTGTCTCAGAAGCTGATATCTT
CTGTACCACTACTGGTAACAAAGATATTATTATGGTTGATCACATGAGGAAGATGAAGAA
CAATGCCATTGTCTGTAACATTGGTCATTTTGACAATGAAATCGACATGCTTGGACTTGA
GACCTACCCTGGTGTGAAACGTATTACCATCAAGCCACAGACCGATAGGTGGGTGTTTCC
TGATACCAACTCTGGCATCATTGTTCTAGCTGAAGGTCGTTTGATGAACTTGGGATGCGC
CACTGGACACCCGAGTTTCGTGATGTCGTGCTCATTCACCAACCAGGTGATTGCACAGCT
TGAGCTCTGGAACGAGAAGTCAACCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGACAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAGTA
CATGTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 16-K019821-022-139-A12C-8409
GenBank Accession LN493235 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K019821-022-139-A12C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGTTGACTTCTC
GTTCCAGAGCTCAAGCTGTGCAATCACCTGGTTGGTGAATGAGCACGACATCACGAAACT
CGGGTGTCCAGTGGCGCATCCCAAGTTCATCAAACGACCTTCAGCTAGAACAATGATGCC
AGAGTTGGTATCAGGAAACACCCACCTATCGGTCTGTGGCTTGATGGTAATACGTTTCAC
ACCAGGGTAGGTCTCAAGTCCAAGCATGTCGATTTCATTGTCAAAATGACCAATGTTACA
GACAATGGCATTGTTCTTCATCTTCCTCATGTGATCAACCATAATAATATCTTTGTTACC
AGTAGTGGTACAGAAGATATCAGCTTCTGAGACAACATCCTCAAGGGTCAGAACTTGAAG
ACCTTCCATAAGAGCCTGAAGAGCACAGATTGGATCAATCTCAGTCACTATGACACGAGC
ACCCGCGGTTTTCATTGCTGCAGCACAACCTTTTCCTACATCACCATAACCACAGATAAC
AGCAACCTTTCCAGCAATCATAACATCAGT

Last Updated on 06.12.2018 16:11