Sequences from confirmation amplicon


Confirmation sequences for line 140G03 and hit At4g29660 are shown.
Line ID 140G03
Locus At4g29660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14534751
Primers used for PCR RK44 - 3144, show primer details
   
Sequence Name 51-K015972-022-140-G03C-3144
GenBank Accession LN507337 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K015972-022-140-G03C-3144
AGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAA
ACTATCAGTGTTTAAACATGGTCTTAGTATCTAATCTAATTCATTGTTCTGTAGTATATT
GTATTCTTTGTCAATGTGTTTCTTACACTGTTTTGTGTTAGTATCAGTTATGTTTCTTGT
TTGAGTGTGAACTTTATGTTACTCCACTCTCTGTAAACAGGAGAAGTTTTGGGAAGAGCA
CCCAGGGAAAACAGTGCCTCTGATGAAGCCAGTATTCTATCGAGGACCATGGAGAGTTTA
TCGTGGTGAGGCTATTGCTTCAGATGCTAGCAGCCAGTGAAGCTAACGATTAGGCTATCA
GAGA
   
Sequence Name 52-K015972-022-140-G03C-RK44
GenBank Accession LN507658 [GenBank]
Sequencing primer gene specific primer, RK44: 5'-CCTTCACCATAGTCTCTGATAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K015972-022-140-G03C-rk44
CGTTACTTCCTGGCTGCTAGCATCTGAAGCAATAGCCTCACCACGATAAACTCTCCATGG
TCCTCGATAGAATACTGGCTTCATCAGAGGCACTGTTTTCCCTGGGTGCTCTTCCCAAAA
CTTCTCCTGTTTACAGAGAGTGGAGTAACATAAAGTTCACACTCAAACAAGAAACATAAC
TGATACTAACACAAAACAGTGTAAGAAACACATTGACAAAGAATACAATATACTACAGAA
CAATGAATTAGATTAGATACTAAGACCATGTTTAAACACTGATAGTTTAAACCGAAGGCG
GGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTAT
ATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAG
ATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATG
CTCCTCGTGGGTGGG
Primers used for PCR 55vo - 3144, show primer details
   
Sequence Name 49-K117876-0022-140-G03C-55vo
GenBank Accession LN506972 [GenBank]
Sequencing primer gene specific primer, 55vo: 5'-TTATATCTGCGTATCAAAGTCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K117876-0022-140-G03C-55vo
TATCGTTAGCTTCACTGGCTGCTAGCATCTGAAGCAATAGCCTCACCACGATAAACTCTC
CATGGTCCTCGATAGAATACTGGCTTCATCAGAGGCACTGTTTTCCCTGGGTGCTCTTCC
CAAAACTTCTCCTGTTTACAGAGAGTGGAGTAACATAAAGTTCACACTCAAACAAGAAAC
ATAACTGATACTAACACAAAACAGTGTAAGAAACACATTGACAAAGAATACAATATACTA
CAGAACAATGAATTAGATTAGATACTAAGACCATGTTTAAACACTGATAGTTTAAACCGA
AGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTC
CTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAG
TGGAGATATCACATCAATCCAC
   
Sequence Name 25-K117875-0022-140-G03C-3144
GenBank Accession LN497645 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117875-0022-140-G03C-3144
CCTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAAACATGGTCTT
AGTATCTAATCTAATTCATTGTTCTGTAGTATATTGTATTCTTTGTCAATGTGTTTCTTA
CACTGTTTTGTGTTAGTATCAGTTATGTTTCTTGTTTGAGTGTGAACTTTATGTTACTCC
ACTCTCTGTAAACAGGAGAAGTTTTGGGAAGAGCACCCAGGGAAAACAGTGCCTCTGATG
AAGCCAGTATTCTATCGAGGACCATGGAGAGTTTATCGTGGTGAGGCTATTGCTTCAGAT
GCTAGCAGCCAGTGAAGCTAACGATTAGGCTATCAGAGACTATGGTGAAGGATGTATCTC
CGACTTTGATACCCAGATATA

Last Updated on 06.12.2018 16:11