Sequences from confirmation amplicon


Confirmation sequences for line 140H07 and hit At1g04980 are shown.
Line ID 140H07
Locus At1g04980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1415833
Primers used for PCR ML13 - 8474, show primer details
   
Sequence Name 35-K100820-0022-140-H07C-8409
GenBank Accession LN501747 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K100820-0022-140-H07C-8409
GCCATTTACAATTGAATTATCCATTTACATGCACTGCTGATCCTGCCAACCATTTCCAAG
GAGAACATTAAACGAAATTAACACATTACGTATCACTACATACGTAATTAAAATTATATA
TAACACTTAAGCCGAAGCTATACACTGCTAAACGAATAAAGAGAAGATCATATAAATCAC
GGTCACGGACATACCTTAGACTTGAAATTAGAAGGAGTGAGTTGAAGCACAGGCGACGAA
GATCCATAGAGAGCATTACCGCGATCGAACAAGGCAAACAACAGACAGCAAATTGGGAAC
ACCGTTGATTTGTACATTTTTCTTTCCATAGTTTCTTCCCTCTCTTTCTTCTCCACCAAA
ACACAAAAAAGTGACTCTTTAGGACACGAACTAAAAACATTGTCTACGTGGACTTACGTG
TACGCACCAATGATAT
   
Sequence Name 35-K100832-0022-140-H07C-ML13
GenBank Accession LN501749 [GenBank]
Sequencing primer gene specific primer, ML13: 5'-AATATCATTGGTGCGTACACGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K100832-0022-140-H07C-ML13
CACTTTTTTGTGTTTTGGTGGAGAAGAAAGAGAGGGAAGAAACTATGGAAAGAAAAATGT
ACAAATCAACGGTGTTCCCAATTTGCTGTCTGTTGTTTGCCTTGTTCGATCGCGGTAATG
CTCTCTATGGATCTTCGTCGCCTGTGCTTCAACTCACTCCTTCTAATTTCAAGTCTAAGG
TATGTCCGTGACCGTGATTTATATGATCTTCTCTTTATTCGTTTAGCAGTGTATAGCTTC
GGCTTAAGTGTTATATATAATTTTAATTACGTATGTAGTGATACGTAATGTGTTAATTTC
GTTTAATGTTCTCCTTGGAAATGGTTGGCAGGATCAGCAGTGCATGTAAATGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
C

Last Updated on 06.12.2018 16:11