Sequences from confirmation amplicon


Confirmation sequences for line 142B04 and hit At5g46340 are shown.
Line ID 142B04
Locus At5g46340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18799508
Primers used for PCR MP18 - 8474, show primer details
   
Sequence Name 17-K024393-022-142-B04C-8409
GenBank Accession LN493694 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K024393-022-142-B04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATTAAAAGTCAATTTACATGCCT
AACTTTTGAAGATTAAAAGTCACTCTCTTAGGTTTCCTCCTACTAAAAGTGGCGTCTTTC
ACTAAACTACAGTCATAATTCCTTCCAGTTAAAGTCTCAACCTTTTCGAATAAATCAACC
TAACCGACTCAACATTGAATCGAAAATTCAGTTCGAGTGTCAAGTTTTCTGGAGAAATTG
TACCTGGCCCGGAGTAATTGGTCCAGGATCCACCATTTTTAACGATCTCTATTCAAAACT
TCCGAACTGCAGACACACCAAATAAGAAGAGCAAATTTATGTAAATCGAAAGAGAAATTT
AAAAGAAACCATTCAGAAAAATTTCAGAAGCAAAATCTTAAAGATTTGAAATTACCAGAG
AGAGATTGAGACGTTAACCGACGAATAAAGAAGATGATCTGGTTATAGTGATCTCTAGTA
GCAGACGATCATCATAAATATGAGAGAGAGAGAGAGATGTGAAAGTGTTTGCTTGAAGAA
GAGAGAAAGACGTTCGGCGAGTTGTCGTTTTCGATCGCCGGCGACTGATTTTTTTCCGAG
TCTCTGGTTAGAGACACTGACTCGA
   
Sequence Name 18-K024393-022-142-B04C-MP18
GenBank Accession LN494142 [GenBank]
Sequencing primer gene specific primer, MP18: 5'-AGACGGAAATATTGTCGAGTCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K024393-022-142-B04C-mp18
TCTAACCAGAGACTCGGAAAAAAATCAGTCGCCGGCGATCGAAAACGACAACTCGCCGAA
CGTCTTTCTCTCTTCTTCAAGCAAACACTTTCACATCTCTCTCTCTCTCTCATATTTATG
ATGATCGTCTGCTACTAGAGATCACTATAACCAGATCATCTTCTTTATTCGTCGGTTAAC
GTCTCAATCTCTCTCTGGTAATTTCAAATCTTTAAGATTTTGCTTCTGAAATTTTTCTGA
ATGGTTTCTTTTAAATTTCTCTTTCGATTTACATAAATTTGCTCTTCTTATTTGGTGTGT
CTGCAGTTCGGAAGTTTTGAATAGAGATCGTTAAAAATGGTGGATCCTGGACCAATTACT
CCGGGCCAGGTACAATTTCTCCAGAAAACTTGACACTCGAACTGAATTTTCGATTCAATG
TTGAGTCGGTTAGGTTGATTTATTCGAAAAGGTTGAGACTTTAACTGGAAGGAATTATGA
CTGTAGTTTAGTGAAAGACGCCACTTTTAGTAGGAGGAAACCTAAGAGAGTGACTTTTAA
TCTTCAAAAGTTAGGCATGTAAATTGACTTTTAATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATT

Last Updated on 06.12.2018 16:11