Sequences from confirmation amplicon


Confirmation sequences for line 143D07 and hit At1g16780 are shown.
Line ID 143D07
Locus At1g16780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5740466
Primers used for PCR RK85 - 3144, show primer details
   
Sequence Name 03-K021851-022-143-D07C-3144
GenBank Accession LN487296 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K021851-022-143-D07C-3144
ATCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA
CAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGA
TGCACCAAAACCATATCCCATAAGATTCGGTTTAAATGGGGATCATCATTTAACTACGTT
TGCTAATGTATCTTTTAAAGAGGTACATTTTTTACTCTTTGGTGTATTCTGTTTCCTCTT
ATTCCATTCCACTCGCATACTCATCCTGATTCTTGTCCTCACATCTAAACAGGTTGATAT
TGCGATCCCAGAAGTCTTCATTGGAGGGTTATTAGGTGCCATGCTTATATTCCTGTTTAG
CGCTTGGGCTTGTGCAGCAGTTGGTCGAACTGCACAGGAGGTTGTCAACGAAGTAAGAAG
ACAGTTCATTGAGAGGCCTGGCATAATGGTAAATGTAATTCCGCTGGAGTCCGTTCCCTT
TTATTTTTATTTTTTTCATCGGGTGTCTTTTTGAACTTTATGTCACAGGACTACAAGGAG
AA
   
Sequence Name 04-K021851-022-143-D07C-RK85
GenBank Accession LN487738 [GenBank]
Sequencing primer gene specific primer, RK85: 5'-CCATAATCTGGCTTCTCCTTGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K021851-022-143-D07C-RK85
AAAATAAAAATAAAAGGGAACGGACTCCAGCGGAATTACATTTACCATTATGCCAGGCCT
CTCAATGAACTGTCTTCTTACTTCGTTGACAACCTCCTGTGCAGTTCGACCAACTGCTGC
ACAAGCCCAAGCGCTAAACAGGAATATAAGCATGGCACCTAATAACCCTCCAATGAAGAC
TTCTGGGATCGCAATATCAACCTGTTTAGATGTGAGGACAAGAATCAGGATGAGTATGCG
AGTGGAATGGAATAAGAGGAAACAGAATACACCAAAGAGTAAAAAATGTACCTCTTTAAA
AGATACATTAGCAAACGTAGTTAAATGATGATCCCCATTTAAACCGAATCTTATGGGATA
TGGTTTTGGTGCATCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATC
CCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGC
GAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACT
TGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGG
Primers used for PCR FL20 - 8474, show primer details
   
Sequence Name 45-K028351-022-143-D07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K028351-022-143-D07C-8409
CATTGCTCAAGGTTCCCATTGTAGGTACAACTGTTTCAAATGGACAACCCGTGGGGGTTC
TCT
   
Sequence Name 45-K028351-022-143-D07C-FL20
GenBank Accession LN505411 [GenBank]
Sequencing primer gene specific primer, FL20: 5'-TGAATTTCTTCATGTGTGGCTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K028351-022-143-D07C-FL20
TATACTGACTATAAGTATGAGCCTGTTCGGACGTTGGCTCTTGCTAGCTCCACTGGTCAT
GGAACCAATATAATTGCTGGGGTCAGCTTGGGTCTGGAATCCACGGCTCTTCCTGTTTTG
GTTATAAGTGTAGCTATCATTTCTGCTTTTTGGCTGGGCAATACCTCGGGACTAATAGAT
GAAAAGGGAAACCCCACTGGAGGTCTATTTGGAACAGCTGTAGCTACAATGGGAATGTTG
AGCACTGCAGCTTATGTTCTTACAATGGACATGTTTGGTCCCATAGCTGATAATGCAGGT
GGGATTGTTGAGATGAGCCAGCAGGTATGAGTCATGAGTGGTTCGAATTATGCCTATGTT
GTATCTTTGAATTTTCAAGCTTCTATAAAACTTATCGTGTCTTGCAGCCAGAAAGTGTCC
GTGAATACCACAAAAGAGGTACATTTGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11