Sequences from confirmation amplicon


Confirmation sequences for line 143E10 and hit K9D7 are shown.
Line ID 143E10
Locus K9D7 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17535626
Primers used for PCR KB81 - 8474, show primer details
   
Sequence Name 20-K100851-0022-143-E10C-8409
GenBank Accession LN495133 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K100851-0022-143-E10C-8409
ATTTACAATTGAATAATCCTGATAGTTAAACCGAAGGCGATGTCACAAGAATACAGTAAT
TCTCCATCATGTATAAAAGTTACAAGAATTGTATAAGGAGATGTCTCAGTCTCATAGGTG
TGTATAGTTTCCTAATGCTTTCACTTATAGTTCACGTATCAACGGGTTTTGCCTGAGTTT
GGAAACTTTCTTTTATCCCATTGGACAATTACAGTAGTGTTCACAAAGATCAAAACCTTT
CTGTATAACAAATCAGGTCCAAACCAAATCATACATTTAGAATTTCCATATCAATCTATA
AGATTCAGAAAACCTGACAATATAAGGTGTAGATAATGACTAAATACCAATATTAATTGT
TTGTATAACCCAATTAGTCAAAACCATTACTGGTACCGA
   
Sequence Name 20-K100857-0022-143-E10C-KB81
GenBank Accession LN495134 [GenBank]
Sequencing primer gene specific primer, KB81: 5'-ATCGGTACAGGTAATGGTTTTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K100857-0022-143-E10C-KB81
CATTATATTGGTATTAGTCATTATCACACCTTATATTGTCAGGTTTTCTGAATCTTATAG
ATTGATATGGAAATTCTAAATGTATGATTTGGTTTGGACCTGATTTGTTATACAGAAAGG
TTTTGATCTTTGTGAACACTACTGTAATTGTCCAATGGGATAAAAGAAAGTTTCCAAACT
CAGGCAAAACCCGTTGATACGTGAACTATAAGTGAAAGCATTAGGAAACTATACACACCT
ATGAGACTGAGACATCTCCTTATACAATTCTTGTAACTTTTATACATGATGGAGAATTAC
TGTATTCTTGTGACATCGCCTTCGGTTTAAACTATCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
Primers used for PCR KB82 - 3144, show primer details
   
Sequence Name 21-K100851-0022-143-E10C-3144
GenBank Accession LN495581 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K100851-0022-143-E10C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGGTA
AATTCGTTGGGAAAAGAGGAGAAAGTCAAACTGATTCATGAGAAGTTAAGAAAGCCCAAG
CCCATTAAATTGAGTATCACTTAATTCGTTATTTATACTAAAAAAGATCGTTTTCTAGAA
TCGAAGTTGGTAGTGTAAAAGAAAAAGAAGTTGAAGAAGCAAGAATTTTCCATCGAAGTG
GTCAATTATCCGAGTGTAGTAAAGATAAAATCTCTTATCAGAAAATAAAATAATCGAAGT
TGGTCAATATTTTTAGTCAAGTCATTCACACGAGGAAATTTCCCACCCAAGAGTTAGGAT
ACGTGGAAAGAAAATCTCAATGACTAATGTGTTACAATAATTAGACGAAGAATAAACTAA
TGCCACACACACACACGCACAAATTCCACTTGTGATGAC
   
Sequence Name 21-K100857-0022-143-E10C-KB82
GenBank Accession LN495582 [GenBank]
Sequencing primer gene specific primer, KB82: 5'-AAGTTCATCAGTCATCACAAGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K100857-0022-143-E10C-KB82
TCTTCGTCTAATTATTGTAACACATTAGTCATTGAGATTTTCTTTCCACGTATCCTAACT
CTTGGGTGGGAAATTTCCTCGTGTGAATGACTTGACTAAAAATATTGACCAACTTCGATT
ATTTTATTTTCTGATAAGAGATTTTATCTTTACTACACTCGGATAATTGACCACTTCGAT
GGAAAATTCTTGCTTCTTCAACTTCTTTTTCTTTTACACTACCAACTTCGATTCTAGAAA
ACGATCTTTTTTAGTATAAATAACGAATTAAGTGATACTCAATTTAATGGGCTTGGGCTT
TCTTAACTTCTCATGAATCAGTTTGACTTTCTCCTCTTTTCCCAACGAATTTACCAAACA
CTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTC
CAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGC
GTCATCCCTTACGTCAGTGGAGATATCA

Last Updated on 06.12.2018 16:11