Sequences from confirmation amplicon


Confirmation sequences for line 144E08 and hit At3g09520 are shown.
Line ID 144E08
Locus At3g09520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2925773
Primers used for PCR KB57 - 3144, show primer details
   
Sequence Name 29-K100851-0022-144-E08C-3144
GenBank Accession LN499205 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K100851-0022-144-E08C-3144
CAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGA
TCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTAATGAAAAATAGTTTGTT
GCGTAACAATTTTGGCATGAAAGGTTAACTACTAATTAATTAGTGTTTGCTTTTACATTT
AAGACGATTATAGACTCTCCTTCACTTTAGGACATGGATTGCCTTGACCGACAAGAGGAA
GGAGAAGAGGCTGACGAATTCCCCGAACTACCCTTCTCTCTGAACAAATCAGACAAATAG
TTTTCAATATCTTCAGGGGTAAATCGGACAACTGAGTTCAGGTTTCTCGCGCCATCGCCT
TCTCCCAAGATAACAGAACCTCTCGTGTTGTAAAACTCGCGATAGATCGGCACCAGCTTT
CTCGATATCGATACTTTGATCTCGTCTCGTATATTTGGATCAGCTACGACGCAGACACTG
TGCTTACTATACGCGTTTTCGAAGCTCTCGCTGAATTTTTCAAACCGCTCCTTCACTTCC
TCCGGCGTCATCTCCACCGTACGATTCTCAGGT
   
Sequence Name 29-K100857-0022-144-E08C-KB57
GenBank Accession LN499206 [GenBank]
Sequencing primer gene specific primer, KB57: 5'-GGTTGCTACTTTACCTGAGAATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K100857-0022-144-E08C-KB57
ACGGTTTGAAAATTCGCGAGAGCTTCGAAAACGCGTATAGTAAGCACAGTGTCTGCGTCG
TAGCTGATCCAAATATACGAGACGAGATCAAAGTATCGATATCGAGAAAGCTGGTGCCGA
TCTATCGCGAGTTTTACAACACGAGAGGTTCTGTTATCTTGGGAGAAGGCGATGGCGCGA
GAAACCTGAACTCAGTTGTCCGATTTACCCCTGAAGATATTGAAAACTATTTGTCTGATT
TGTTCAGAGAGAAGGGTAGTTCGGGGAATTCGTCAGCCTCTTCTCCTTCCTCTTGTCGGT
CAAGGCAATCCATGTCCTAAAGTGAAGGAGAGTCTATAATCGTCTTAAATGTAAAAGCAA
ACACTAATTAATTAGTAGTTAACCTTTCATGCCAAAATTGTTACGCAACAAACTATTTTT
CATTAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCT
GTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGG
GATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
Primers used for PCR KB56 - 8474, show primer details
   
Sequence Name 49-K100997-0022-144-E08C-8409
GenBank Accession LN506769 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K100997-0022-144-E08C-8409
CGACTATAAGTGAAGATGCTATGTTTTATTTTTTATTTTTCCTTACAGTTTAAGGTAAAA
ATCTTCGGGTTTCTATTTCGAACATTATGTCACACATTTGTCTGTAATGTTTAATTAATG
ATATATCTATAAAAAGATCGTCATGTTTCTCATTTTTCAAAAATGTATTTGTAAGTAAGA
GACACGTGTACGGAAAAGATGAGTCAAAGGCCAACGGCACATTCTTCCCCATATTTCGCT
GACGTTGTTTTGTAACCATAACGATNAACGACCTAAATCTTTCATTATGGTTGCGTAACA
GATTAAAAATATCTACAGTTTCTGTTCTAACGTAACGACGCCTCCCTGCATTTGGATTAA
GTGGTAGGTTCTTTAAAAGGGAACCCTTTTCACCACTAGTTCTTGAATCCACATACAAGC
CGAGGCGTTTCAATATTAAATGTAAATTTTACCCTTAAAACCATTTGATTTCTCCTTCAA
AATTCCTGCAACGACNTTAATTTGAAAAAAAAAAAACAGAGACTTAAGATCAAAGATAAG
GAGCAAGAGCGGTTTTAGTTTCTTCTCTAAAATGACTTCTTCAAGGAAAAGGCCTTCTC
   
Sequence Name 52-K101001-0022-144-E08C-KB56
GenBank Accession LN507752 [GenBank]
Sequencing primer gene specific primer, KB56: 5'-TTGCTAGGTAGTGAGAGAGAAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K101001-0022-144-E08C-KB56
TTTGAGAGAACTAAACCGCTCTTGCTCCTCATCTTTGATCTTACGTCTCTGTTTTTTTTT
TTTAAATTAATGTCGTTGCAGGAATTTTGAAGGAAAAATCAAATGGTTTTAAGGGTAAAA
TTTACATTTAATATTGAAACCCCTCGGCTTGTATGTGGATTCAAAAACTAGGGGTGAAAA
GGGTTCCCTTTTAAAGAACCTACCACTTAATCCAAATGCAGGGAGGCGTCTTTACGTTAA
AACAAAAACTGTAAATTTTTTTAATCTGTTACCCAACCATAATGAAAGATTTAGGTCGTT
TATCGTTATGGTTACAAAACAACGTCAGCAAAATATGGGGAAAAATGTGCCGTTGGCCTT
TGACTCATCTTTTCCGTACACGTGTCTCTAACTTACAAATACTTTTTTGAAAAATGAAAA
ACATGACAATTTTTTTATAGATTTATCATTAATTAAACTTTACAGACAAATGTGTGACAT
AATGTTCGAAATAAAAACCCGAAAATTTTTACCTTAAACTGTAGGGAAAAATAAAAAATA
AAACATAGCTTTCTTCACTTTATATGTCGTTGTCCGGGAAATCTACTTGGATCACCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTCCCAATTTTTTTTCAATTCAAA
AATGTAAATT

Last Updated on 06.12.2018 16:11