Sequences from confirmation amplicon


Confirmation sequences for line 144H03 and hit At4g30130 are shown.
Line ID 144H03
Locus At4g30130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14736313
Primers used for PCR 03um - 8474, show primer details
   
Sequence Name 62-K117671-0022-144-H03C-03um
GenBank Accession LN510842 [GenBank]
Sequencing primer gene specific primer, 03um: 5'-AGCAGATAGTTTCTTTGGGATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K117671-0022-144-H03C-03um
TATACCGCCTCCTTCACCACAGAACTCTCAATGGGATTTCTTTTGGAATCCATTCTCGGC
ATTGGATCAGTATGGATACAGTTATGATAACCAAAATGGTATGGATGATGATATGAGACG
GCTGAGGCGGGTTCGGGAAGAAGAAGGGATTCCAGATCTTGAAGAAGATGAATATGTAAA
GTTTGAGGATCATCATAACATGAAAGCAACAGAAGATTTTAATGGAGGTAAAATGTATCA
AGAAGATAAAGTAGAACATGTTAATGAGGAGTTCACGGACAGTGGATGCAAGATTGAGAA
TGAGAGCGACAAGAACTGCAATGGAACTCAAGAACGGCGCAGTCTTGAGGTGTCCAGAGG
AGGAACTACAGGGCATGTTGTTGGAGTTACATCAGATGATGGAAAAGGAGAGACACCTGG
TTTCACTGTGTACTTGAATAGAAGGCCAACAAGCAATGAGTATGATGGTCATGGCTTCAT
GTCCGGGAAATTTACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCA
   
Sequence Name 14-K117670-0022-144-H03C-8409
GenBank Accession LN492693 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117670-0022-144-H03C-8409
CATTTACATTGTAAATTTCCCGGACATGAAGCCATGACCATCATACTCATTGCTTGTTGG
CCTTCTATTCAAGTACACAGTGAAACCAGGTGTCTCTCCTTTTCCATCATCTGATGTAAC
TCCAACAACATGCCCTGTAGTTCCTCCTCTGGACACCTCAAGACTGCGCCGTTCTTGAGT
TCCATTGCAGTTCTTGTCGCTCTCATTCTCAATCTTGCATCCACTGTCCGTGAACTCCTC
ATTAACATGTTCTACTTTATCTTCATGATACATTTTACCTCCATTAAAATCTTCTGTTGC
TTTCATGTTATGATGATCCTCAAACTTTACATATTCATCTTCTTCAAGATCTGGAATCCC
TTCTTCTTCCCGAACCCGCCTCAGCCGTCTCATATCATCATCCATACCATTTTGGTTATC
ATAACTGTATCCATACTGATCCAATGCCGAGAATGGATTCCAAAAGAAATCCCATTGAGA
GATCTGTGGTGAAGGAGGCGGTATATTGTGACTGCCTAGTCCCGGACTGTTCATGTTCAT
GTTCATCCCAAAGAAACTATCTGCT

Last Updated on 06.12.2018 16:11