Sequences from confirmation amplicon


Confirmation sequences for line 144H10 and hit At5g42000 are shown.
Line ID 144H10
Locus At5g42000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16799846
Primers used for PCR 04qw - 8474, show primer details
   
Sequence Name 35-K115837-0022-144-H10C-04qw
GenBank Accession LN501957 [GenBank]
Sequencing primer gene specific primer, 04qw: 5'-AGAAGAAAGAAAACTTGCTCAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K115837-0022-144-H10C-04qw
TCTCGATCTTCTTCAATCCCTCCGGTGAAAAGACGATGTACGTAAGAGCACTTCCGACGA
CGGATGTGAATCGGAACACAGAGTGGTTCACATATCCTGGTGTTTGGACTACTTATATTC
TCATCCTCTTCTTCTCTTGGCTCCTCGTTCTCTCCGTCTTCCATTGTTCTCCTGGCATCG
CTTGGACCATTGTTCATCTCGCTCATTTCACCGTATTATTCCCGATCTCCTCCGATCCAA
TTTCGAAATTGTTGGTTTCTAGATTTGAATTTTTGAGTTTTTTTGTTTTCTAGGTCACGT
ATCATTCCTTCCATTGGAAGAAGGGAACACCATTTGGAGATGATCAAGGAGTCTATAATA
GATTGACTTGGTGGGAACAGATTGATAATGGCAAGCAGCTTACACGTAATCGCAAATTTC
TCACCGTAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCA
   
Sequence Name 03-K115833-0022-144-H10C-8409
GenBank Accession LN487627 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115833-0022-144-H10C-8409
TTACGGTGAGAATTTGCGATTACGTGTAAGCTGCTTGCCATTATCAATCTGTTCCCACCA
AGTCAATCTATTATAGACTCCTTGATCATCTCCAAATGGTGTTCCCTTCTTCCAATGGAA
GGAATGATACGTGACCTAGAAAACAAAAAAACTCAAAAATTCAAATCTAGAAACCAACAA
TTTCGAAATTGGATCGGAGGAGATCGGGAATAATACGGTGAAATGAGCGAGATGAACAAT
GGTCCAAGCGATGCCAGGAGAACAATGGAAGACGGAGAGAACGAGGAGCCAAGAGAAGAA
GAGGATGAGAATATAAGTAGTCCAAACACCAGGATATGTGAACCACTCTGTGTTCCGATT
CACATCCGTCGTCGGAAGTGCTCTTACGTACATCGTCTTTTTCACCGGAGGGATTTGGAA
GAAGATCGGAGATGATGATTGATGAATCGTCGTTGAGCAAGTTTCTTTTCTTC

Last Updated on 06.12.2018 16:11