Sequences from confirmation amplicon


Confirmation sequences for line 145B10 and hit At2g23760 are shown.
Line ID 145B10
Locus At2g23760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10109482
Primers used for PCR JU04 - 8474, show primer details
   
Sequence Name 35-K019823-022-145-B10C-JU04
GenBank Accession LN501645 [GenBank]
Sequencing primer gene specific primer, JU04: 5'-CTAATGGATATTTGAAGCCAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K019823-022-145-B10C-JU04
TAAATAATTAATGTGTACAAGACTTAGAGGTCTGCTAGTTAACATTGAAATGAAAGCTTC
TAACTTTCATGTACTTGAGAAATACTTACATCTCTCTCTCCTATGTTTTATATATCCTTG
TGTGTGTAAGTGTAAATAATGTATTTAATGAAAAAAACGAACCCCAAGAAAAACAAATGA
TTCGTGATATTGAAAATCCATGCAAGAGAATAGACATGAAGCCACTCATGTCATATTTTG
CATGATGAAATGATTGATATATATGGTAAATTGTGAGTTAAGTACATATATGTAAATGAG
TAATTAAAAGGGTTCTTGTATGATAAGTATGAAAACATATTATATTAAAAAAATATTGAT
GTATGTATCTATATAGATAAAGGCTGACCTGATTTCTGGATAAACCAGTCTGTCGTGCTA
AGAGGTGCTTATCAGCATCGCTTGGGTACCTGAGAAAGAGACCAGAAAAAGAAACAAACG
AGGGATATGCATCAGCAATCTACATGGATCAGCAATGAGTATGATGGTCNATATGGAGAA
AAAGAAAGAGTAATTACCTTTTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
   
Sequence Name 36-K019823-022-145-B10C-8409
GenBank Accession LN502044 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K019823-022-145-B10C-8409
CATGTAGATTGCTGATGCATATCCCTTGTTTGTTTCTTTTTCTGGTCTCTTTCTCAGGTA
CCCAAGCGATGCTGATAAGCACCTCTTAGCACGACAGACTGGTTTATCCAGAAATCAGGT
CAGCCTTTATCTATATAGATACATACATCAATATTTTTTTAATATAATATGTTTTCATAC
TTATCATACAAGAACCCTTTTAATTACTCATTTACATATATGTACTTAACTCACAATTTA
CCATATATATCAATCATTTCATCATGCAAAATCTGACATGAGTGGCTTCATGTCTATTCT
CTTGCATGGATTTTCAATATCACGAATCATTTGTTTTTCTTGGGGTTCGTTTTCTTCATT
AAATACATTATCTACACTTACACACACAAGGATATATAAAACATAGGAGAGAGAGATGTA
AGTATTTCTCAAGTACATGAAAGTTTGAAGCTTTCATTTCAATGTTAACTAGCAGACCTC
TAAGTCTTGTACACATTAATTATTTACATCACAAGTAGTTTATTACACTTGGCTTCAAAT
ATTCCATT

Last Updated on 06.12.2018 16:11