Sequences from confirmation amplicon


Confirmation sequences for line 145C05 and hit At3g03200 are shown.
Line ID 145C05
Locus At3g03200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:736458
Primers used for PCR MZ03 - 8474, show primer details
   
Sequence Name 50-K016441-022-145-C05C-8409
GenBank Accession LN507013 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K016441-022-145-C05C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAACCCTTGATA
ATGAAGACGTGAATACAAATGAAACCCTTGATTCATCGGGATTCGAGGTGGTTGAAGAAG
AAACTAGATTTAACAACCAAATGCTCATCTCGACATATCAAACGACAAAGATTCTATATC
ACCAAGTCGTACCTTGTCACACGTTGAAAGTTCACGTCAATCCTATTAGTCACAATGTGG
AAGAGAGAACATTGTTCATTGAAGAGGACAAAGATTCTTGGTTACAAAGAGCTGAGAAGA
TCACGAAGACAAAACTAACACTTTTTAGTTTAATGGCTCAGCAATACTACAAATGTCTTG
CTATTTTTTTCTGATTCTTTTCTTTTTAAATTTGTTGATTTATTTATCCCTTTTTGATAA
AAGGGCATTTACTTAGACACATATATGTATGTATACTAATATTTACATTTAACTGTTGTA
CTTCTTACTGTTAAAAAATATTTCTTATACCTATAACAACAATAAATTAGACGATCCAAA
TTTAAATTAGTATATCATCAAATATTTGAATGGA
   
Sequence Name 54-K016441-022-145-C05C-MZ03
GenBank Accession LN508321 [GenBank]
Sequencing primer gene specific primer, MZ03: 5'-TGACGCTTGTACTCCATTCAAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K016441-022-145-C05C-mz03
TTGATGATATACTAATTTAAATTTGGATCGTCTAATTTATTGTTGTTATAGGTATAAGAA
ATATTTTTTAACAGTAAGAAGTACAACAGTTAAATGTAAATATTAGTATACATACATATA
TGTGTCTAAGTAAATGCCCTTTTATCAAAAAGGGATAAATAAATCAACAAATTTAAAAAG
AAAAGAATCAGAAAAAAATAGCAAGACATTTGTAGTATTGCTGAGCCATTAAACTAAAAA
GTGTTAGTTTTGTCTTCGTGATCTTCTCAGCTCTTTGTAACCAAGAATCTTTGTCCTCTT
CAATGAACAATGTTCTCTCTTCCACATTGTGACTAATAGGATTGACGTGAACTTTCAACG
TGTGACAAGGTACGACTTGGTGATATAGAATCTTTGTCGTTTGATATGTCGAGATGAGCA
TTTGGTTGTTAAATCTAGTTTCTTCTTCAACCACCTCGAATCCCGATGAATCAAGGGTTT
CATTTGTATTCACGTCTTCATTATCAAGGGTTTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA

Last Updated on 06.12.2018 16:11