Sequences from confirmation amplicon


Confirmation sequences for line 145E02 and hit At4g33060 are shown.
Line ID 145E02
Locus At4g33060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15949970
Primers used for PCR JK34 - 8474, show primer details
   
Sequence Name 07-K024844-022-145-E02C-8409
GenBank Accession LN489097 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K024844-022-145-E02C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTAGATAGTTTTTTAA
CTTTATGGTTGTCGTTCTTTATTTTAACAAGCAATCTTGACTTTCTGATGTATTTTTATA
TTTCAGAACGCATCTGAGTCCAAGGAGGTACTATCTGTGCGAGAAGCTCTGAATGCTAAG
AAAGAAGCGGCTCAAAAAGATAAAAGCTTTTCTGTATCTGATACAGTTGGAAATAGTGAT
GATGACGATGATGGTGAAGATGAGACTAAATTTGATGCAAAGATGAGGAATCAAGTGCTC
AGCAGAAGGAAGGAGATTGGAGATACGCCTTCAAAGCCGACTCAGAAAAAAAGTAATTAC
GTTTTCCATCTCTCTATATCCTGATATCCTGAAATATTAAACTTATGGCAATTCTTGATT
TGAATAATCAAATCTTCAATCATATTAAGCAATCATGTCTATGTTTAGGGATGTTATCTG
TTTATCAACACGATTTGATGCTTTTAGGTTTGGCACCAAGTATTGCCAGAAATAATATAC
AGTTCTCTCCTCATGCGTGTTCTTTACATTATCTCTAGTTATGTCTGTATATTCTAATTA
TAATATACTTGTGGTTGTTTTCTTAACAGAGAGCTCTAGCCTGAAAGGCCGTGAAGAGTC
TACGCAAAGGTAATGACACCCACAGTTATACGCTACGTCTCTGT
   
Sequence Name 08-K024844-022-145-E02C-JK34
GenBank Accession LN489561 [GenBank]
Sequencing primer gene specific primer, JK34: 5'-TGGAAGACAAATGATACAGAGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K024844-022-145-E02C-JK34
GCGTATAACTGTGGGTGTCATTACCTTTGCGTAGACTCTTCACGGCCTTTCAGGCTAGAG
CTCTCTGTTAAGAAAACAACCACAAGTATATTATAATTAGAATATACAGACATAACTAGA
GATAATGTAAAGAACACGCATGAGGAGAGAACTGTATATTATTTCTGGCAATACTTGGTG
CCAAACCTAAAAGCATCAAATCGTGTTGATAAACAGATAACATCCCTAAACATAGACATG
ATTGCTTAATATGATTGAAGATTTGATTATTCAAATCAAGAATTGCCATAAGTTTAATAT
TTCAGGATATCAGGATATAGAGAGATGGAAAACGTAATTACTTTTTTTCTGAGTCGGCTT
TGAAGGCGTATCTCCAATCTCCTTCCTTCTGCTGAGCACTTGATTCCTCATCTTTGCATC
AAATTTAGTCTCATCTTCACCATCATCGTCATCATCACTATTTCCAACTGTATCAGATAC
AGAAAAGCTTTTATCTTTTTGAGCCGCTTCTTTCTTAGCATTCAGAGCTTCTCGCACAGA
TAGTACCTCCTTGGACTCAGATGCGTTCTGAAATATAAAAATACATCAGAAAGTCAAGAT
TGCTTGTTAAAATAAAGAACGACAACCATAAAGTTAAAAAACTATCTAATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATT

Last Updated on 06.12.2018 16:11