Sequences from confirmation amplicon


Confirmation sequences for line 145H10 and hit At1g21770 are shown.
Line ID 145H10
Locus At1g21770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7652263
Primers used for PCR KB40 - 3144, show primer details
   
Sequence Name 40-K100996-0022-145-H10C-3144
GenBank Accession LN503643 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K100996-0022-145-H10C-3144
TCCCCTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAACG
AAGATCGTGTGGAACGAAGGAAGACATAGATTCGAGACTGATGATCACGAAGCCTTCATA
GAGTACAAGATGAAGAATGACGGTNAAGTCATGGATCTGGTTCGAACCTTCGTACCGCCG
TCAAAAGCAGGACTTGGTTTAGCTTCGCATCTATGCGTCGCCGCTTTCGAACACGCCTCT
GAACACTCTTTCTCCATCATCCCCACTTGCTCCTACGTTTCCGTATACATCTCTTCTTTC
CTCAAAAAATTGAGATGAATCAGATTCTTATGAAAATATGTCGTGTTTTGCAGGAAACGT
TTCTTCATCGAAACCCAACGTGGCAGCGTCTGGTTCACTCAGAGGATGATTCCNAGAACC
TCANGTCTAGCATCTGATCAAGTCCTGCTTTAAAGCCTTTTGGTGTATAATAAAAACTTG
AAAACATTATCTTACAATAGTAAAGCTTGTTTCAAGTAATCTCTGTAAACAAACAAAAAT
CCCAAAATGTATGTTTTTCACAATTCGACAG
   
Sequence Name 40-K101000-0022-145-H10C-KB40
GenBank Accession LN503644 [GenBank]
Sequencing primer gene specific primer, KB40: 5'-ATTAGTGTTTGTTTGTTGCTGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K101000-0022-145-H10C-KB40
TTTTGGGATTTTTGTTTGTTTACGAGATTACTTGAAACAAGCTTTACTATTGCAAGATAA
TGTTTTCAAGTTTTTATTATACACCAAAAGGCTTTAAAGCAGGACTTGATCAGATGCTAG
ACTTGAGGTTCTTGGAATCATCCTCTGAGTGAACCAGACGCTGCCACGTTGGGTTTCGAT
GAAGAAACGTTTCCTGCAAAACACGACATATTTTCATAAGAATCTGATTCATCTCAATTT
TTTGAGGAAAGAAGAGATGTATACGGAAACGTAGGAGCAAGTGGGGATGATGGAGAAAGA
GTGTTCAGAGGCGTGTTCGAAAGCGGCGACGCATAGATGCGAAGCTAAACCAAGTCCTGC
TTTTGACGGCGGTACGAAGGTTCGAACCAGATCCATGACTTTACCGTCATTCTTCATCTT
GTACTCTATGAAGGCTTCGTGATCATCAGTCTCGAATCTATGTCTTCCTTCGTTCCACAC
GATCTTCGTTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGG
GTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGG
ATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
Primers used for PCR FK28 - 8474, show primer details
   
Sequence Name 10-K101267-0022-145-H10C-8409
GenBank Accession LN490581 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K101267-0022-145-H10C-8409
TCGGAATGAGCCATTTACATTGTTTCTCTAGTCTAAGTCCACTCTCTTTCATTTCAACCG
AGAATTGATTTTAATATATCGTTAGTAAACCGGTTTAATGGATAAAAACGTATAATCGTC
GTACACGTGGCAGGCGATGGAGGCGGCAGAGTGTTTTGCTTGCTTCGTCGTGTTATTTGG
CGTGAAGTAAAGAGTTCAGAGAGAAAAGGAAGTCAAAACTTCAGAGAGAAAGTGGATGGG
GGACACGAAGGTAGAGACAATCTCGAGGTTAGCTCAATGGAGAATTGAAAATTTTGGTCC
TTGTTCTTTCAAAAAGTCCGATCCTTTCAAAGTCGGAATTTGGAATTGGTAAGCTTTTCA
TTTTTGGTTTATTTTGGTAAATTTTCTTCGGAATTTTGATTTTTGTTTGTTTTGGGATAT
GATGAAGGCATCTGTCTATAGAGAGGAATCGCTATCTATCAGTGAGGCTATTCCCAGAAC
TTTCTCGAGTATCTAAAGAGCAACCTCCTGTTGCCAAATTTGTTCTTCGTGTCTCCAACG
TTGGCCCCAATCGTAGATTCTACATCTCTCCTGGTATGTCTTGTCTTTGAATTGACTTTT
CTGGATTTGGAATTTGAATTCAC
   
Sequence Name 10-K101273-0022-145-H10C-FK28
GenBank Accession LN490582 [GenBank]
Sequencing primer gene specific primer, FK28: 5'-GAATTCAAATTCCAAATCCAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K101273-0022-145-H10C-FK28
GATGTAGAATCTACGATTGGGGCCAACGTTGGAGACACGAAGAACAAATTTGGCAACAGG
AGGTTGCTCTTTAGATACTCGAGAAAGTTCTGGGAATAGCCTCACTGATAGATAGCGATT
CCTCTCTATAGACAGATGCCTTCATCATATCCCAAAACAAACAAAAATCAAAATTCCGAA
GAAAATTTACCAAAATAAACCAAAAATGAAAAGCTTACCAATTCCAAATTCCGACTTTGA
AAGGATCGGACTTTTTGAAAGAACAAGGACCAAAATTTTCAATTCTCCATTGAGCTAACC
TCGAGATTGTCTCTACCTTCGTGTCCCCCATCCACTTTCTCTCTGAAGTTTTGACTTCCT
TTTCTCTCTGAACTCTTTACTTCACGCCAAATAACACGACGAAGCAAGCAAAACACTCTG
CCGCCTCCATCGCCTGCCACGTGTACGACGATNATACGTTTTTATCCATTAAACCGGTTT
ACTAACGATATATTAAAATCAATTCTCGGTTTGAAATGAAAGAGAGTGGACTTAGACTTA
GAGAAACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTA
GATGTCCG

Last Updated on 06.12.2018 16:11