Sequences from confirmation amplicon


Confirmation sequences for line 146E09 and hit At3g06010 are shown.
Line ID 146E09
Locus At3g06010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1804542
Primers used for PCR DF16 - 8474, show primer details
   
Sequence Name 21-K029122-022-146-E09C-8409
GenBank Accession LN495537 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K029122-022-146-E09C-8409
ATCCATGTAGATTTCCCGGACATAAATTGGTTCACTCAATTTTCTTTAAAAATGCTTTAT
CTCTCATGATAAGATCATAATGGGTTATCAACACATTAAATTTTCCTTCTCCTGCAATTT
TTTCCCTTATTGCTTTCCTTTCCTCTAAACGTCCATCATACAGAAAAGCTGCAATGCTGT
CAAAAAAACAAAAAGGCTGATTTTCAGACATAACATTCATACCAGTGCAATTTCTCAGTA
CCAAGAGAAATATTGTTTGCAACATACCTTGGTACCCATGTTGCAAACTCGTTTACCCAA
TTTGGTAGCACAGCTTTTGGAGCAACTATAAGATAAGGCCCAGGCACACCTTTATTCTCC
AGAAGATACGCAATCAGAGAAATTGTTTGAATAGTTTTTCCCAAA
   
Sequence Name 22-K029122-022-146-E09C-DF16
GenBank Accession LN495982 [GenBank]
Sequencing primer gene specific primer, DF16: 5'-GGTTTGGGAAAAACTATTCAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K029122-022-146-E09C-DF16
ATTTCTCTGATTGCGTATCTTCTGGAGAATAAAGGTGTGCCTGGGCCTTATCTTATAGTT
GCTCCAAAAGCTGTGCTACCAAATTGGGTAAACGAGTTTGCAACATGGGTACCAAGGTAT
GTTGCAAACAATATTTCTCTTGGTACTGAGAAATTGCACTGGTATGAATGTTATGTCTGA
AAATCAGCCTTTTTGTTTTTTTGACAGCATTGCAGCTTTTCTGTATGATGGACGTTTAGA
GGAAAGGAAAGCAATAAGGGAAAAAATTGCAGGAGAAGGAAAATTTAATGTGTTGATAAC
CCATTATGATCTTATCATGAGAGATAAAGCATTTTTAAAGAAAATTGAGTGAACCAATTT
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11