Sequences from confirmation amplicon


Confirmation sequences for line 146F12 and hit MUF9 are shown.
Line ID 146F12
Locus MUF9 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24308290
Primers used for PCR KR57 - 3144, show primer details
   
Sequence Name 82-K100823-0022-146-F12C-3144
GenBank Accession LN514151 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K100823-0022-146-F12C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAGA
TACTCTTGTATTGATCATATGTGGATACTCTTGTATTGTTCTTACAATTTTTTTATTTAT
TTTTAGATTGATATCTAATACATACTAGATATTTGATGATGATTTAATCTTTTTTAAGGA
AAATAAATATTAGAGTATACTGCACGGATTAATTAAATAGAATGATGTGACATAGATTTA
GCCTTTTTTTCAACTAAAAGTTAACCTTAATTTTATTATATTTTCCTTAAATTTAACATT
AATTTTATTATATTTTCTATATTAGAATTAGATTTTTATCATTTTAGGAATATTTCACAA
TATCTGTATTTCATCATTATAAGGAATGAAATTAGAGGGAAAGCCA
   
Sequence Name 82-K100835-0022-146-F12C-KR57
GenBank Accession LN514153 [GenBank]
Sequencing primer gene specific primer, KR57: 5'-AATGGCTTTCCCTCTAATTTCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K100835-0022-146-F12C-KR57
TGTGAATATTCCTANATGATAAAATCTAATTCTAATATAGAAAATATAATAAAATTAATG
TTAAATTTAAGGAAAATATAATAAAATTAAGGTTAACTTTTAGTTGAAAAAAAGGCTAAA
TCTATGTCACATCATTCTATTTAATTAATCCGTGCAGTATACTCTAATATTTATTTTCCT
TAAAAAAGATTAAATCATCATCAAATATCTAGTATGTATTAGATATCAATCTAAAAATAA
ATAAAAAAATTGTAAGAACAATACAAGAGTATCCACATATGATCAATACAAGAGTATCTC
AAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTC
CTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGAT
TGTGCGTCATCCCTTACGTCAGTGGAGATATCA
Primers used for PCR 26wp - 3144, show primer details
   
Sequence Name 27-K118534-0022-146-F12C-26wp
GenBank Accession LN498606 [GenBank]
Sequencing primer gene specific primer, 26wp: 5'-AACGGGTATGATGATCTCCTTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118534-0022-146-F12C-26wp
CTTCTCGGGATCCTGAAAAAGATGGAGGATCTTATATACTGATAGTGAGAACGATATGAT
GGTCGTTGGCGATGATCCATGGCAGTAAGTTCTTTATACATACCTTCTAAAGGTCAATCA
CAACTTCTTTTCTCCTAACTTGAAATTGGCTTTCTGCTATGGATAGTGATTTCTGCAATG
TGGTGTGGAAGATACACTTATACACGAAAGAGGAAGTGGAGAATGCGAATGACGATAACA
AGAGTTGTTTAGAGCAAGCTGCTCTCATGATGGAAGCATCAAAGTCATCTTCTGTGAGCC
AGCCTGATTCTTCTCCTACAATCACTAGGGTTTGATACCCATAAAGAAGCTTATTTCCTA
TGTTTTAAAGTGTGTTTTGCTCACAAAAGAACTTCAACTTTATCTTTGTCTTTGAATCCA
TTTATGTGTTTGTTTGTGTTTCTTCTGGTCTCCATGGATGTCTCATGTGTACCGTTTTAC
TCGAGAGATATGTGAGTTTATGGGATGTGTAAAGCATGCCATTGGATTTTAAGGTTTTCA
AAATTACAATATATATATTAGTTTTGAAGTATTTTGCTTGATTTTGAAAATTTCATTTCT
TAAACACTGATATTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCC
CCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCG
AAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
   
Sequence Name 06-K118533-0022-146-F12C-35St
GenBank Accession LN489015 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K118533-0022-146-F12C-35St
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAATATCAGTGTTTA
AGAAATGAAATTTTCAAAATCAAGCAAAATACTTCAAAACTAATATATATATTGTAATTT
TGAAAACCTTAAAATCCAATGGCATGCTTTACACATCCCATAAACTCACATATCTCTCGA
GTAAAACGGTACACATGAGACATCCATGGAGACCAGAAGAAACACAAACAAACACATAAA
TGGATTCAAAGACAAAGATAAAGTTGAAGTTCTTTTGTGAGCAAAACACACTTTAAAACA
TAGGAAATAAGCTTCTTTATGGGTATCAAACCCTAGTGATTGTAGGAGAAGAATCAGGCT
GGCTCACAGAAGATGACTTTGATGCTTCCATCATGAGAGCAGCTTGCTCTAAACAACTCT
TGTTATCGTCATTCGCATTCTCCACTTCCTCTTTCGTGTATAAGTGTATCTTCCACACCA
CATTGCAGAAATCACTATCCATAGCAGAAAGCCAATTTCAAGTTAGGAGAAAAGAAGTTG
TGATTGACCTTTAGAAGGTATGTATAAAGAACTTACTGCCATGGATCATCGCCAACGACC
ATCATATCGTTCTCACTATCAGTATATAAGATCCTCCATCCTTTTTCAGGATCCCTGAGA
AGCCCTTCCATGTTGAACAGCCGTTCAAGCTCCATAAGGAG

Last Updated on 06.12.2018 16:11