Sequences from confirmation amplicon


Confirmation sequences for line 147D08 and hit At5g41900 are shown.
Line ID 147D08
Locus At5g41900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16770054
Primers used for PCR AY01 - 8474, show primer details
   
Sequence Name 18-K015115-022-147-D08C-AY01
GenBank Accession LN494089 [GenBank]
Sequencing primer gene specific primer, AY01: 5'-TTGAATTCTACCGTGATTGAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K015115-022-147-D08C-AY01
AGCTCCAAGATGGTCGGATTGCCATTGCAGTTTCTGCACTTCTTGGCTTACTTCTACCAA
CAGAGATTCTCTGTTTGTTAAAGTTCAACAACCCAAAGGTAATTAATTTATCCACTTCCT
ACATTTATTTGTATGTATATTATATATATACACACAAACAAATATAAATGAAATAAATGA
TTCAAAGGTTATTTGTTGTAAAATGCTAACATTAGCTTAAAAAAAATCAGCAATTACAAA
AATAAATTTTCTTACAAGAATTATAACATTTTTACTAGTCTATATCAAATTATATGATTC
AAAGGGTTCTACATTATTTGTTGTTACGTAAAAAGTACTAGAATTTAATATACTCATTAC
TATATTTTACTTGTCGCTATCCGATATCGGATACACAACAGTTTTCTTATTTTAGGTAAT
ATATTAAGGTAACATATAAAATAAATGAAATATATGTAAAATATGTGCAGATAACAAGAA
GGCCCGAGACAACGTTGTGTTTATACATGGTTTTGTATCATCATCCGCGTTTTGGACGGA
GACTCTCTTCCCAAACTTTTCTGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
AT
   
Sequence Name 17-K015115-022-147-D08C-8409
GenBank Accession LN493648 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K015115-022-147-D08C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATCAGAAAAGTTTGGGAAGAGAGTC
TCCGTCCAAAACGCGGATGATGATACAAAACCATGTATAAACACAACGTTGTCTCGGGCC
TTCTTGTTATCTGCACATATTTTACATATATTTCATTTATTTTATATGTTACCTTAATAT
ATTACCTAAAATAAGAAAACTGTTGTGTATCCGATATCGGATAGCGACAAGTAAAATATA
GTAATGAGTATATTAAATTCTAGTACTTTTTACGTAACAACAAATAATGTAGAACCCTTT
GAATCATATAATTTGATATAGACTAGTAAAAATGTTATAATTCTTGTAAGAAAATTTATT
TTTGTAATTGCTGATTTTTTTTAAGCTAATGTTAGCATTTTACAACAAATAACCTTTGAA
TCATTTATTTCATTTATATTTGTTTGTGTGTATATATATAATATACATACAAATAAATGT
AGGAAGTGGATAAATTAATTACCTTTGGGTTGTTGAACTTTAACAAACAGAGAATCTCTG
TTGGTAGAAGTAAGCCAAGAAGTGCAGAAACTGCAATGGCAATCCGACCATCTTGGAGCT
GTCGAAGATTTCTCAATCA

Last Updated on 06.12.2018 16:11