Sequences from confirmation amplicon


Confirmation sequences for line 148C12 and hit At4g28490 are shown.
Line ID 148C12
Locus At4g28490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14079427
Primers used for PCR IS42 - 8474, show primer details
   
Sequence Name 09-K021056-022-148-C12C-8409
GenBank Accession LN489982 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K021056-022-148-C12C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCATGTAGAGTTGATTCT
TACTGAGATCAAGCCTGCTCAATTGCTTCAATTTCACCAAGCTTTCAGGAATCTCACCGG
AGAAATCATTCTCCGCTCCGGAAATCTCGATTATTCCGTTAAGCGATCCGATTTCGTTGG
GGATTGAACCTGAAAATCGATTCTTTGAGATTCGTAGGTTCGATAGATTCTTCGCGCCGA
TGATCGTCTTAGGAATACTTCCGGTGAACGAATTGTCAGAGAGTTCGAGAAGTGACAAGC
GAGGCAATCCCCAGAATCCATGTGGGATTTGACCCGAAAGCTTGTTGTTACTCAATCGGA
CCCGGGTCAAGCTCTTACACTTTCCGAGATTATTAGAGATTTCTCCTGAAAACGAATTGT
CTATAAGAATTAGATACTCTAGCTTCCCTTCACCGCAGACATTCGCCGGTATTTCGCCGG
AAAATCGGTTATACGATAAGTCCACGTACTGTAACGGTGAGTTAGCGCCGAGTTGACTCG
GTAATACTCCGGTGAGTCTGTTGTTGAA
   
Sequence Name 10-K021056-022-148-C12C-IS42
GenBank Accession LN490455 [GenBank]
Sequencing primer gene specific primer, IS42: 5'-GAACTCAAGCTTTTCAACAACAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K021056-022-148-C12C-IS42
TCACCGGAGTATTACCGAGTCAACTCGGCGCTAACTCACCGTTACAGTACGTGGACTTAT
CGTATAACCGATTTTCCGGCGAAATACCGGCGAATGTCTGCGGTGAAGGGAAGCTAGAGT
ATCTAATTCTTATAGACAATTCGTTTTCAGGAGAAATCTCTAATAATCTCGGAAAGTGTA
AGAGCTTGACCCGGGTCCGATTGAGTAACAACAAGCTTTCGGGTCAAATCCCACATGGAT
TCTGGGGATTGCCTCGCTTGTCACTTCTCGAACTCTCTGACAATTCGTTCACCGGAAGTA
TTCCTAAGACGATCATCGGCGCGAAGAATCTATCGAACCTACGAATCTCAAAGAATCGAT
TTTCAGGTTCAATCCCCAACGAAATCGGATCGCTTAACGGAATAATCGAGATTTCCGGAG
CGGAGAATGATTTCTCCGGTGAGATTCCTGAAAGCTTGGTGAAATTGAAGCAATTGAGCA
GGCTTGATCTCAGTAAGAATCAACTCTACATGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAA

Last Updated on 06.12.2018 16:11