Sequences from confirmation amplicon


Confirmation sequences for line 148H03 and hit At4g27830 are shown.
Line ID 148H03
Locus At4g27830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13862985
Primers used for PCR VL15 - 3144, show primer details
   
Sequence Name 09-K017369-022-148-H03C-VL15
GenBank Accession LN489963 [GenBank]
Sequencing primer gene specific primer, VL15: 5'-CATTGGTTCTTATGACCAAGGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K017369-022-148-H03C-vl15
CTAACAAATTCATCAATTGTACTAGTGGAAATTCTTCTACAGAACCATATCTTGCAGGCC
ATAACATATTGCTAGCTCATGCCTCTGCTTCAAAGTTGTATAAACTAAAGTACAAGGTAT
ATACACACATTTTTTGCTCTTAAAACCTGAACCTCGTATGATCATAGTTCATTATGATGG
TGCAGAGTACGCAGAAAGGATCCATTGTAAATGCATCTTGTGGTTAAGTTGATCAGCAAT
GATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCA
AATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGT
CATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAA
CGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGG
   
Sequence Name 13-K017369-022-148-H03C-3144
GenBank Accession LN491823 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K017369-022-148-H03C-3144
GATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTT
CATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAAC
TATCATTGCTGATCAACTTAACCACAAGATGCATTTACAATGGATCCTTTCTGCGTACTC
TGCACCATCATAATGAACTATGATCATACGAGGTTCAGGTTTTAAGAGCAAAAAATGTGT
GTATATACCTTGTACTTTAGTTTATACAACTTTGAAGCAGAGGCATGAGCTAGCAATATG
TTATGGCCTGCAAGATATGGTTCTGTAGAAGAATTTCCACTAGTACAATTGATGAATTTG
TTAGGAGAACAATGTCCAGGGGGCGAGATTCCTTGGTCATAA
Primers used for PCR 58wg - 8474, show primer details
   
Sequence Name 21-K118262-0022-148-H03C-58wg
GenBank Accession LN495855 [GenBank]
Sequencing primer gene specific primer, 58wg: 5'-CCATCGGCATACCTGTTTTTATATT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118262-0022-148-H03C-58wg
GAACAGAAGTGTGCTTTCAAGCAGACACAACAGAGTAAGAAAACAAAAGAGAATGAAGTG
TTGTACCATTTTCAAGAATATAGATTGGAGGATTGTTATAGCTCTGCTTTATATACTCAA
GAATACCTTCAAGACCCCATGGAGTAGCCTCCCACTTCAAGAAAAACAATTGATTCAAGA
AGAGCTTTTGGCAAAAAATATATATGTATTATGCAAATTTGCAGGACCTTACCAGAAGAA
ATGAAGAATTCGCAGCGGCTGTCCAAAGAAGAAGAACTATTAGTTTTAAGAATTGATCAG
AAGCATTTTTCTATTGGTTAATGATGAAAGAAAACTAATCTTACAAATCATATATACTCC
CATGTCTTTAAAGAAGCCTTCATTCATGCTGGGAAAGATAGAAGGTGAGGGTTTGTTTGT
GACATAAAATGTCGTGTAGTGAATAATTCCTATAAAGTCAGATGATCCTTTAAGTTGCTC
TGACTCTTCCTCCGAAAACACCGGTAATCTCGATCCAACAGTTCTCTTCATTTCATCCGG
ATAGTCTCCAAATACCAAAGGCTTCAACATCCTGAATTATCATTTTAGTCTTCAGATTTT
GTCCGCAAGTTATTGAAACACAGAGAAAGAAAGCTTTCTTTTTGCTCACCAGCCATAGAA
GAAAGCTTTAGCTCTNTGAGTTGCGATTTCATCATCCTTGGAGTTTGTATATGGAGATAA
CCCGAATGCAAATATGTGTAAATGGCAATCTACATTCTCCGGGGTGTAAATTGTAAATGG
TCTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAA
   
Sequence Name 01-K118261-0022-148-H03C-8409
GenBank Accession LN486775 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118261-0022-148-H03C-8409
ATTTACATTGAATATATCCTGGAGACCATTTACAATTTACAACCCGGAGAATGTAGATTG
CCATTTACACATATTTGCATTCGGGTTATCTCCATATACAAACTCCAAGGATGATGAAAT
CGCAACTCAAAGAGCTAAAGCTTTCTTCTATGGCTGGTGAGCAAAAAGAAAGCTTTCTTT
CTCTGTGTTTCAATAACTTGCGGACAAAATCTGAAGACTAAAATGATAATTCAGGATGTT
GAAGCCTTTGGTATTTGGAGACTATCCGGATGAAATGAAGAGAACTGTTGGATCGAGATT
ACCGGTGTTTTCGGAGGAAGAGTCAGAGCAACTTAAAGGATCATCTGACTTTATAGGAAT
TATTCACTACACGACATTTTATGTCACAAACAAACCCTCACCTTCTATCTTTCCCAGCAT
GAATGAAGGCTTCTTTAAAGACATGGGAGTATATATGATTTGTAAGATTAGTTTTCTTTC
ATCATTAACCAATAGAAAAATGCTTCTGATCAATTCTTAAAACTAATAGTTCTTCTTCTT
TGGACAGCCGCTGCGAATTCTTCATTTCTTCTGGTAAGGTCCTGCAAATTTGCATAATAC
ATATATATTTTTTGCCAAAAGCTCTTCTTGAATCAATTGTTTTTCTTGAAGTGGGAGGCT
ACTCCATGGGGTCTTGAAGGTATTCTTGAGTATATAAAGCAGAGCTATAACAATCCTCCA
ATCTATATTCTTGAAAATGGTACAACACTTCATTCTCTTTTGTTTTCTTACTCTGTTGTG
TCTGCTTGAAAGCACACTTTTCTGTTCTTTAAGATTCTTCAACTTGCAATTTTTTAATAT
AAAA

Last Updated on 06.12.2018 16:11