Sequences from confirmation amplicon


Confirmation sequences for line 149F01 and hit At5g19530 are shown.
Line ID 149F01
Locus At5g19530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6590583
Primers used for PCR MR37 - 8474, show primer details
   
Sequence Name 31-K015723-022-149-F01C-8409
GenBank Accession LN499938 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K015723-022-149-F01C-8409
TAGATTTCCCCGACATGAAGCCATTTACAATTGAATATATCCCGCTCTCCTTTTCCATCC
CAAGTATGCACTTTATTTTACTTCACTTACAAATAAAATTTTGTATATATAAAAGATTTT
TTAGTAATCAAATTGAGTACTTCAGATTTGAACAGTGCACCTATATAGCCTACTGATTCT
ACTAATATTTAGTAAATTATATCACTTGTTTATTATTATACATGAACATTTTATATAAAC
ATAGTTTGGAATTGTCATTTACTTAACATTTGCTCATTGAGTAGCCCCAAGACTGTGTTT
ATAATGGGAGGAGGTGAAGGCTCTGCTGCAAGAGAAATACTAAAACACACGACGATCGAG
AAAGTTGTTATGTGTGATATTGATCAGGTTAATCTCATACCTCCAATTGTATTAACAAAA
ATATACGTTCATGCATGATCTTAACAAATACATGTTTTTTCTTTCTTCTTCTAGGAAGTT
GTTGATTTTTGCAGAAGATTTCTGACCGTTAACAGCGATGCTTTCTGTAACAAAAAGCTT
GAACTTGTGATCAAAGATGCAAAGTAAGGAACCTAAAACAAAACAAAACAAAAAAAGTTT
TTATCTGACGTTTAAAATCTCTAAAAAATATAATTTGAAATGGGAAAAAAG
   
Sequence Name 32-K015723-022-149-F01C-MR37
GenBank Accession LN500362 [GenBank]
Sequencing primer gene specific primer, MR37: 5'-CGAAACAGGCTTTAATTTGCTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K015723-022-149-F01C-MR07
TTGCTTCATGGTGTTGTAGATTGATGTGAAGACTTCCTTATGAGTGAATATTCCTGCTGG
TCCAGCCTATGAATGATTTAAGTAAGTAAATTTAGTCTTTTGTTATCATTCTTCTAAAAT
TATTAATATTTATCAAAGAATAATTAGAAGAAAAAATAAAAGATGTATAGACCTGGGTGA
CAAAAATGCCATTAGGGCTAAGCTTGGGTTTGAGAATGTTTTGGTAGAAGGATTTGGTGT
AGAGCTGATAACAAGGTCCACCTTCCACTGGATCAGCTAAATCTCCCACTATGATATCAA
ACTTCTCTTCCCTTTTCTCTAATTCAGCCCTTTTTCACATTTCAAATTATATTTTTTAGA
GATTTTAAACGTCAGATAAAAACTTTTTTTGTTTTGTTTTGTTTTAGGTTCCTTACTTTG
CATCTTTGATCACAAGTTCAAGCTTTTTGTTACAGAAAGCATCGCTGTTAACGGTCAGAA
ATCTTCTGCAAAAATCAACAACTTCCTAGAAGAAGAAAGAAAAAACATGTATTTGTTAAG
ATCATGCATGAACGTATATTTTTGTTAATACAATTGGAGGTATGAGATTAACCTGATCAA
TATCACACATAACAACTTTCTCGATCGTCGTGTGTTTTAGTATTTCTCTTGCAGCAGAGC
CTTCACCTCCTCCCATTATAAACACAGTCTTGGGGCTACTCAATGAGCAA

Last Updated on 06.12.2018 16:11