Sequences from confirmation amplicon


Confirmation sequences for line 150C10 and hit At5g40540 are shown.
Line ID 150C10
Locus At5g40540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16238269
Primers used for PCR NB97 - 8474, show primer details
   
Sequence Name 27-K035540-022-150-C10C-NB97
GenBank Accession LN498290 [GenBank]
Sequencing primer gene specific primer, NB97: 5'-GTTCTGAATCCATTATTGTTTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K035540-022-150-C10C-NB97
TTGAGCTTGTGTCTTAATCTTTGCATGTGTGAAAAATTGAAACTTTTTTGCTTCAATATA
TCTAGTCATTTGCATATCACTATATCAGTTTCAGTTTAATGGTTGTTGTTCAATGAGTTC
AGAATAAAAAGAATTACTATGTTTGTATCAAAAATTTCAGAGAAATCTTTTCTCTAGAAA
TTTCAGAGCATTAGTGTGAATCTGTGGAGCGAATTCAATCATAAAGATAGTTTTCATAGA
TGTTATAGTTTGATGAAGATTGAAGAGTATTGAGAATGATGTAAAATGTGGTTTCTTGTT
TAGGTATAAGAACAAAACAGTTGCTATTAAGATTGTTAAAAGAGGAGAATCTCCCGAAGA
GATTGCGAAAAGAGAGAGCAGATTTGCAAGAGAGTATGATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 12-K035540-022-150-C10C-8409
GenBank Accession LN491483 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K035540-022-150-C10C-8409
TTGCAAATCTGCTCTCTCTTTTTCGCAATCTCTTCGGGAGATTCTCCTCTTTTAACAATC
TTAATAGCAACAGTTTTGTTCTTATACCTAAACAAGAAACCACATTTTACATCATTCTCA
ATACTCTTCAATCTTCATCAAACTATAACATCTATGAAAACTATCTTTATGATTGAATTC
GCTCCACAGATTCACACTAATGCTCTGAAATTTCTAGAGAAAAGATTTCTCTGAAATTTT
TGATACAAACATAGTAATTCTTTTTATTCTGAACTCATTGAACAACAACCATTAAACTGA
AACTGATATAGTGATATGCAAATGACTAGATATATTGAAGCAAAAAAGTTTCAATTTTTC
ACACATGCAAAGATTAAGACACAAGCTCAAGAAGACCAGAACCAGTCCAAACAGATCAAT
CGCCGAGAAAAAGGGAAACAA
Primers used for PCR AE08 - 8474, show primer details
   
Sequence Name 43-K118809-0022-150-C10C-AE08
GenBank Accession LN505011 [GenBank]
Sequencing primer gene specific primer, AE08: 5'-TGGATGACTCCATGAGAGTGTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118809-0022-150-C10C-AE08
GCATATCACAGCTACCGTATGTCCAACTCCCTGGACGCAAGCTAACAAGGTATTTACGCA
ATGTACCACCGAGTAAAAGCTCGGTGACTATAACCATGATTGGTTCTTTGCAAGCTCCAA
TGAACTACAAAACATACCAAATCAAAACGTTTTGGTGTTTGTCTCTAACCAAATTATGTA
GCAATATGTACTGGAGATTAGATACATGGATCAGCAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCACTATTATAAT
   
Sequence Name 11-K118808-0022-150-C10C-8409
GenBank Accession LN491341 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118808-0022-150-C10C-8409
TGATCCATGTATCTAATCTCCANTACATATTGCTACATANATTTGGTTAGAGACAAACAC
CAAAACNGTTTTGATTTGGTATGTTTTGTAGTTCATTGGAGCTTGCAAAGAACCAATCAT
GGTTATAGTCACCGAGCTTTTACTCGGTGGTACATTGCGTAAATACCTTGTTAGCTTGCG
TCCAGGGAGTTTGGACATACGTGTAGCTGTTGGATATGCCCTTGACATTGCTCGGGCGAT
GGAATGCTTACACTCTCATGGAGTCATCC

Last Updated on 06.12.2018 16:11