Sequences from confirmation amplicon


Confirmation sequences for line 151C10 and hit At5g43170 are shown.
Line ID 151C10
Locus At5g43170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17331220
Primers used for PCR NR28 - 8474, show primer details
   
Sequence Name 60-K036418-022-151-C10C-NR28
GenBank Accession LN510187 [GenBank]
Sequencing primer gene specific primer, NR28: 5'-AGTGGACCAAATGTAAGAAACGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K036418-022-151-C10C-NR28
AACCACCGTCTCACTGAGGAAGAGTATCTAGCTTTCTGTCTCATGCTTCTTGCTCGGGAT
GGCGGCGATCTTGACTCTGTGACGGTTGCGGAGAAGCCGAGTTATAAGTGTGGCGTTTGT
TACAAGACGTTTTCGTCTTACCAAGCTCTCGGCGGTCATAAAGCGAGCCACCGGAGCTTA
TACGGTGGTGGAGAGAATGATAAATCGACACCATCCACCGCCGTGAAATCTCACGTTTGT
TCGGTTTGCGGGAAATCTTTCGCCACCGGTCAAGCTCTCGGCGGCCACAAGCCATGTATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTACATTCAC
   
Sequence Name 52-K036418-022-151-C10C-8409
GenBank Accession LN507736 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K036418-022-151-C10C-8409
TGAGCCATTTACAATTGAATATACATGGCTTGTGGCCGCCGAGAGCTTGACCGGTGGCGA
AAGATTTCCCGCAAACCGAACAAACGTGAGATTTCACGGCGGTGGATGGTGTCGATTTAT
CATTCTCTCCACCACCGTATAAGCTCCGGTGGCTCGCTTTATGACCGCCGAGAGCTTGGT
AAGACGAAAACGTCTTGTAACAAACGCCACACTTATAACTCGGCTTCTCCGCAACCGTCA
CAGAGTCAAGATCGCCGCCATCCCGAGCAAGAAGCATGAGACAGAAAGCTAGATACTCTT
CCTCAGTGAGACGGTGGTTATGATGAAGATCAGATCTCGAACGTTTGGATCGTTTCTTAC
ATTTGGTCC

Last Updated on 06.12.2018 16:11