Sequences from confirmation amplicon


Confirmation sequences for line 152F02 and hit At3g05160 are shown.
Line ID 152F02
Locus At3g05160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1453970
Primers used for PCR RU50 - 8474, show primer details
   
Sequence Name 05-K024450-022-152-F02C-8409
GenBank Accession LN488187 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K024450-022-152-F02C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGTAATATTATAAGTGCTAATAGTTATC
TTATATTAGTCCAATATTAGGAAACAAGAGTAACCTGTAACGTGAAGCTAACCCCAATAA
GCAAGGATCCGATAGACATTCCAACAGCAGAAGCCTAGAATAATGTTTATCAAGATAATG
TTAATAATTATTATACTGTTATATTGTTATGTCTATATATAAACTGTATTTTTCTAACCA
ATAGGAGTGGTCGTCTTCCCCATCTATCCACAAGAATCAGACCAACCAAAGCTTTTGGGA
TCTACAAAGAGTAAAAAATAATCATAATGGTATAATCGAAATAGCTTTGAGAAATTTAAT
ATCGTAAAAAAAAAATCAATTAGTTAAAGGTACGCACCACGAACACACCAAA
   
Sequence Name 06-K024450-022-152-F02C-RU50
GenBank Accession LN488637 [GenBank]
Sequencing primer gene specific primer, RU50: 5'-GATCAATGATATTTGGTGTGTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K024450-022-152-F02C-RU50
GTGCGTACCTTTAACTAATTGATTTTTTTTTTACGATATTAAATTTCTCAAAGCTATTTC
GATTATACCATTATGATTATTTTTTACTCTTTGTAGATCCCAAAAGCTTTGGTTGGTCTG
ATTCTTGTGGATAGATGGGGAAGACGACCACTCCTATTGGTTAGAAAAATACAGTTTATA
TATAGACATAACAATATAACAGTATAATAATTATTAACATTATCTTGATAAACATTATTC
TAGGCTTCTGCTGTTGGAATGTCTATCGGATCCTTGCTTATTGGGGTTAGCTTCACGTTA
CAGGTTACTCTTGTTTCCTAATATTGGACTAATATAAGATAACTATTAGCACTTATAATA
TTACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATTC

Last Updated on 06.12.2018 16:11